Rapid Detection of Yeast in Orange Juice by Enzyme-linked Immunosorbent Assay
| Autor: | Hisao Maeda, Motonobu Yoshida, Yasushi Ifuku |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
chemistry.chemical_classification
Orange juice Antiserum biology Fungi imperfecti Rhodotorula rubra Candida parapsilosis biology.organism_classification Yeast General Biochemistry Genetics and Molecular Biology Microbiology Enzyme Biochemistry chemistry Candida intermedia General Agricultural and Biological Sciences |
| Zdroj: | Agricultural and Biological Chemistry. 55:2951-2957 |
| ISSN: | 0002-1369 |
| DOI: | 10.1080/00021369.1991.10859821 |
| Popis: | Antisera against native yeast of 6 species, Candida intermedia, C. parapsilosis, C. guilliermondii, C. Iambica, Crytococcus lamentii, and Rhodotorula rubra, were prepared for use as probes in an enzyme-linked immunosorbent assay (ELISA). These antisera reacted with yeast cell surface antigens that are thought to consist of protein moieties. The qross-reactivities between the antisera and yeast of 11 species were investigated by immunofluorescent and immunoblotting methods. It was shown that ELISA using the antiserum against C. intermedia or C. parapsilosis, is a valuable means of detecting yeast in orange juice. Yeast of more than 103 were capable of being detected by ELISA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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