Berberine Activates Aryl Hydrocarbon Receptor but Suppresses CYP1A1 Induction through miR-21-3p Stimulation in MCF-7 Breast Cancer Cells
| Autor: | Chun Wei Wang, I-Jung Lee, Yune-Fang Ueng, Sheng-Nan Lo, Yueh-Shieh Chen, Lih-Ann Li, Chiung-Chiao Huang, Tian Shung Wu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Jatrorrhizine Berberine Berberine Alkaloids CYP1A1 Pharmaceutical Science Estrogen receptor Breast Neoplasms Article Analytical Chemistry lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Organic chemistry Drug Discovery Basic Helix-Loop-Helix Transcription Factors Cytochrome P-450 CYP1A1 Humans Physical and Theoretical Chemistry Goldenseal Messenger RNA biology Dose-Response Relationship Drug Molecular Structure Chemistry aryl hydrocarbon receptor Organic Chemistry Transfection respiratory system biology.organism_classification Aryl hydrocarbon receptor berberine miR-21-3p breast cancer cells Molecular biology Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology MCF-7 Biochemistry Receptors Aryl Hydrocarbon Chemistry (miscellaneous) 030220 oncology & carcinogenesis biology.protein MCF-7 Cells Molecular Medicine Female |
| Zdroj: | Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules, Vol 22, Iss 11, p 1847 (2017) Molecules; Volume 22; Issue 11; Pages: 1847 |
| ISSN: | 1420-3049 |
| Popis: | Berberine and the methylenedioxy ring-opening derivatives palmatine and jatrorrhizine are active ingredients in immunomodulatory plants, such as goldenseal. This study aimed to illustrate the effects of protoberberines on aryl hydrocarbon receptor (AhR) activation and cytochrome P450 (CYP) 1 in the estrogen receptor (ER)α(+) MCF-7 breast cancer cells. Among protoberberines at non-cytotoxic concentrations (≤10 μM), berberine had the most potent and statistically significant effects on AhR activation and CYP1A1/1A2/1B1 mRNA induction. The 24-h exposure to 10 μM berberine did not change CYP1A1 mRNA stability, protein level and function. Berberine significantly increased micro RNA (miR)-21-3p by 36% and the transfection of an inhibitor of miR-21-3p restored the induction of CYP1A1 protein with a 50% increase. These findings demonstrate that the ring opening of the methylenedioxyl moiety in berberine decreased AhR activation in MCF-7 cells. While CYP1A1 mRNA was elevated, berberine-induced miR-21-3p suppressed the increase of functional CYP1A1 protein expression. |
| Databáze: | OpenAIRE |
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