Immunogenicity and Protective Efficacy in Mice of Influenza B Virus Vaccines Grown in Mammalian Cells or Embryonated Chicken Eggs
| Autor: | B. Fanget, Elena A. Govorkova, Shantha Kodihalli, C. Gerdil, Robert G. Webster, Irina V. Alymova |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
viruses
Immunology Viral Pathogenesis and Immunity Hemagglutinin (influenza) Chick Embryo Antibodies Viral Recombinant virus Microbiology Immunoglobulin G Virus Cell Line Mice Dogs Antigen Virology Chlorocebus aethiops Influenza Human Animals Humans Vero Cells Glycoproteins B-Lymphocytes Mice Inbred BALB C HN Protein biology Immunogenicity Genetic Variation Influenza B virus Influenza Vaccines Insect Science biology.protein Vero cell Antibody |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1098-5514 0022-538X |
| Popis: | The immunogenicity and protective efficacy of formalin-inactivated influenza B/Memphis/1/93 virus vaccines propagated exclusively in Vero cells, MDCK cells, or embryonated chicken eggs (hereafter referred to as eggs) were investigated. Mammalian cell-grown viruses differ from the egg-grown variant at amino acid position 198 (Pro/Thr) in the hemagglutinin gene. The level of neuraminidase activity was highest in egg-grown virus, while MDCK and Vero cell-derived viruses possessed 70 and 90% less activity, respectively. After boosting, each of the vaccines induced high levels of hemagglutinin-inhibiting, neuraminidase-inhibiting, and neutralizing antibodies that provided complete protection from MDCK-grown virus challenge. Mammalian cell-derived virus vaccines induced serum antibodies that were more cross-reactive, while those induced by egg-grown virus vaccines were more specific to the homologous antigen. Enzyme-linked immunospot analysis indicated that cell-grown virus vaccines induced high frequencies of immunoglobulin G (IgG)-producing cells directed against both cell- and egg-grown virus antigens, whereas egg-grown virus vaccine induced higher frequencies of IgG- and IgM-producing cells reacting with homologous antigen and low levels of IgG-producing cells reactive with cell-grown viruses. These studies indicate that influenza B virus variants selected in different host systems can elicit different immune responses, but these alterations had no detectable influence on the protective efficacy of the vaccines with the immunization protocol used in this study. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|