Anti-Streptococcal activity of Brazilian Amazon Rain Forest plant extracts presents potential for preventive strategies against dental caries
| Autor: | Ingrit Elida Collantes Díaz, Adriana Lígia de Castilho, Ivana Barbosa Suffredini, Juliana Paola Correa da Silva, Mateus Luís Barradas Paciencia, Cíntia Helena Couri Saraceni |
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| Rok vydání: | 2013 |
| Předmět: |
Colony Count
Microbial Microbial Sensitivity Tests Dental Caries Antioxidants Trees Streptococcus mutans Reference Values Botany Humans General Dentistry Analysis of Variance Plants Medicinal biology Plant Extracts Forestry Plant extracts Original Articles biology.organism_classification Anti-Bacterial Agents lcsh:RK1-715 lcsh:Dentistry Anti-infective agents Chromatography Thin Layer Cintia Amazonian ecosystem Brazil Amazon rain forest |
| Zdroj: | Journal of Applied Oral Science Journal of Applied Oral Science v.22 n.2 2014 Journal of applied oral science Universidade de São Paulo (USP) instacron:USP Journal of Applied Oral Science, Volume: 22, Issue: 2, Pages: 91-97, Published: APR 2014 Journal of Applied Oral Science, Vol 22, Iss 2, Pp 91-97 (2014) |
| ISSN: | 1678-7765 |
| Popis: | Caries is a global public health problem, whose control requires the introduction of low-cost treatments, such as strong prevention strategies, minimally invasive techniques and chemical prevention agents. Nature plays an important role as a source of new antibacterial substances that can be used in the prevention of caries, and Brazil is the richest country in terms of biodiversity. Objective: In this study, the disk diffusion method (DDM) was used to screen over 2,000 Brazilian Amazon plant extracts against Streptococcus mutans. Material and Methods: Seventeen active plant extracts were identified and fractionated. Extracts and their fractions, obtained by liquid-liquid partition, were tested in the DDM assay and in the microdilution broth assay (MBA) to determine their minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs). The extracts were also subjected to antioxidant analysis by thin layer chromatography. Results: EB271, obtained from Casearia spruceana, showed significant activity against the bacterium in the DDM assay (20.67±0.52 mm), as did EB1129, obtained from Psychotria sp. (Rubiaceae) (15.04±2.29 mm). EB1493, obtained from Ipomoea alba, was the only extract to show strong activity against Streptococcus mutans (0.08 mg/mL |
| Databáze: | OpenAIRE |
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