Allogeneic hematopoietic stem cell transplant after COVID-19 infection and its effect on the antibody titers to SARS-CoV-2

Autor: Rachna Seth, Aditya Gupta, Ritu Gupta, Mohanaraj Ramachandran, Urvashi B. Singh, Tanima Dwivedi, Kiran Bala, Uma Kanga, Jagdish Prasad Meena, Aditya Kumar Gupta
Rok vydání: 2021
Předmět:
2019-20 coronavirus outbreak
B Cells
Coronavirus disease 2019 (COVID-19)
Bioinformatics
Cell Transplantation
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Immune Cells
Immunology
chemical and pharmacologic phenomena
Bone Marrow Cells
Surgical and Invasive Medical Procedures
Research and Analysis Methods
White Blood Cells
Database and Informatics Methods
Animal Cells
hemic and lymphatic diseases
Medicine and Health Sciences
Blood and Lymphatic System Procedures
Medicine
Humans
Antibody-Producing Cells
Molecular Biology Techniques
Molecular Biology
Bone Marrow Transplantation
Transplantation
Blood Cells
Comparative Sequence Analysis
business.industry
SARS-CoV-2
Antibody titer
Hematopoietic Stem Cell Transplantation
COVID-19
Biology and Life Sciences
Cell Biology
Virology
surgical procedures
operative
Pediatrics
Perinatology and Child Health
Allogeneic hematopoietic stem cell transplant
Cellular Types
business
Sequence Analysis
Research Article
Cloning
Stem Cell Transplantation
Zdroj: PLoS ONE
ISSN: 1399-3046
Popis: After allogeneic hematopoietic stem cell transplantation (HSCT), recovery of humoral immunity is essential to protect from life-threatening infections. However, monitoring the humoral immune system after transplantation with standard techniques in the clinical routine is imprecise. Here, we performed sequencing of mononuclear bone marrow cells to characterize the VH1-repertoire of switched B cells of healthy volunteers and patients undergoing HSCT. Analysis of healthy bone marrow donors and patients showed virtually no clonally related sequences between individuals. Interestingly, clonally related sequences were present in pre- and post-transplantation bone marrow of patients undergoing HSCT for acute myeloid leukemia treatment. We consistently observed such related B cell clones, irrespective of conditioning regimen, donor source or time post transplantation. In general, repertoire diversity was lower in post-HSCT as compared to pre-HSCT samples. However, post-HSCT repertoires retained highly mutated sequences, despite immunosuppressive therapy and presence of T cell deficiency after HSCT. These observations identify key properties of the recovering B cell compartment and provide a conceptual framework for the surveillance of humoral immunity after allogeneic transplantation.
Databáze: OpenAIRE
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