Advanced non-small cell lung cancer: Rapid evaluation of EGFR status on fine-needle cytology samples using Idylla
| Autor: | Caterina Picariello, Angela D'Ardia, Chiara Ciaparrone, Pio Zeppa, Antonio D'Antonio, Sara Gaeta, Rosalba Fumo, Alessandro Caputo |
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| Přispěvatelé: | D'Ardia, A., Caputo, A., Fumo, R., Ciaparrone, C., Gaeta, S., Picariello, C., Zeppa, P., D'Antonio, A. |
| Rok vydání: | 2021 |
| Předmět: |
FNAC
Oncology Advanced NSCLC EGFR Idylla ROSE medicine.medical_specialty Lung Neoplasms Cytodiagnosis Biopsy Fine-Needle DNA Mutational Analysis Metastatic tumor Pathology and Forensic Medicine Carcinoma Non-Small-Cell Lung Internal medicine Cytology Humans Medicine Mutational status Epidermal growth factor receptor Lung cancer Rapid On-site Evaluation biology business.industry Cell Biology medicine.disease Rapid assessment Aspiration cytology ErbB Receptors Mutation biology.protein Non small cell business |
| Zdroj: | Pathology - Research and Practice. 224:153547 |
| ISSN: | 0344-0338 |
| Popis: | Advanced non-small cell lung cancer (NSCLC) needs to be managed rapidly; therefore, a rapid assessment of the epidermal growth factor receptor (EGFR) status is mandatory. Computed Tomography (CT)-guided or Ultrasound (US)-guided Fine-Needle Aspiration Cytology (FNAC) allows a rapid diagnosis of both primary and metastatic tumor through rapid on-site evaluation (ROSE) and the proper management of diagnostic material. Idylla (Biocartis, Mechelen, Belgium) is an automated RT-PCR system which evaluates the mutational status of specific genes in less than two hours. In this study, the EGFR mutational status in advanced NSCLC was analyzed on 28 FNAC samples with Idylla. After ROSE, residual FNAC material and/or additional passes were pipetted into the Idylla EGFR cartridge. Patients endorsed a consent form before carrying out the analysis. Results were controlled by pyrosequencing. Adequate EGFR status was obtained in 26/28 cases (22 wild type and 4 mutated). Mutated cases harbored EGFR Exon 19 deletion and L858R point mutation. In 2/28 cases the analysis failed. The combination of FNAC, ROSE and Idylla is a rapid, accurate and effective method that can be conveniently used to assess EGFR status in advanced NSCLC. |
| Databáze: | OpenAIRE |
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