Dose-dependent effect of triiodothyronine on the chondrogenic differentiation of mesenchymal stem cells from the bone marrow of female rats
| Autor: | Natália de Melo Ocarino, André Luiz B P Azevedo, Alfredo M. Goes, Greiciane Gaburro Paneto, Jankerle Neves Boeloni, Higor A Assis, Rogéria Serakides, Adriana Madeira Álvares da Silva, Nathalia C Elert, Iolanda S Braga, Francisco de Paula Careta |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Time Factors Dose dependence Pharmaceutical Science SOX9 Matrix (biology) Cell morphology 03 medical and health sciences 0302 clinical medicine Chondrocytes Internal medicine medicine Animals Rats Wistar Collagen Type II Cells Cultured Pharmacology Triiodothyronine Dose-Response Relationship Drug Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells SOX9 Transcription Factor Chondrogenesis Rats 030104 developmental biology Endocrinology medicine.anatomical_structure 030220 oncology & carcinogenesis Female Bone marrow |
| Zdroj: | The Journal of pharmacy and pharmacology. 70(1) |
| ISSN: | 2042-7158 |
| Popis: | ObjectivesVerify the in-vitro effect of triiodothyronine (T3) on the chondrogenic differentiation of female rat bone marrow mesenchymal stem cells (BMMSCs) over several time periods and at several doses.MethodsCD54 + /CD73 + /CD90 + BMMSCs from Wistar female rats were cultured in chondrogenic medium with or without T3 (0.01; 1; 100; 1000 nm). At seven, 14 and 21 days, the cell morphology, chondrogenic matrix formation and expression of Sox9 and collagen II were evaluated.Key findingsThe dose of 100 nm did not alter the parameters evaluated in any of the periods studied. However, the 0.01 nm T3 dose improved the chondrogenic potential by increasing the chondrogenic matrix formation and expression of Sox9 and collagen II in at least one of the evaluated periods; the 1 nm T3 dose also improved the chondrogenic potential by increasing the chondrogenic matrix formation and the expression of collagen II in at least one of the evaluated periods. The 1000 nm T3 dose improved the chondrogenic potential by increasing the chondrogenic matrix formation and Sox9 expression in at least one of the evaluated periods.ConclusionsT3 has a dose-dependent effect on the differentiation of BMMSCs from female rats. |
| Databáze: | OpenAIRE |
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