A comparison of the results of sequential hydrazinolysis-nitrosation and alkali-mediated cleavage-nitrosation of theO-linked oligosaccharides of gastric mucus glycoproteins
| Autor: | Steven R. Carter, J.Michael Williams, John R. Clamp |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Magnetic Resonance Spectroscopy
Swine Nitrosation Molecular Sequence Data Oligosaccharides Hydrazone Cellobiose Alkalies Cleavage (embryo) Biochemistry Analytical Chemistry chemistry.chemical_compound Animals Laminaribiose Glycoproteins chemistry.chemical_classification Chemistry Organic Chemistry General Medicine Nuclear magnetic resonance spectroscopy Alkali metal Glycopeptide Hydrazines Carbohydrate Sequence Gastric Mucosa |
| Zdroj: | Carbohydrate Research. 205:181-190 |
| ISSN: | 0008-6215 |
| DOI: | 10.1016/0008-6215(90)80138-s |
| Popis: | Analysis of the oligosaccharides released from pig gastric mucus glycopolypeptides by hydrazinolysis showed that degradation had occurred. Nitrosation of the products followed by reduction gave a mixture that had a low content of 2,5-anhydro-D-talitol, which implied destruction of much of the terminal reducing 2-amino-2-deoxy-D-galactose. Under the conditions of hydrazinolysis, cellobiose was largely unchanged but laminaribiose gave a complex mixture that probably contained glucose hydrazone (13C-n.m.r. data). In order to avoid degradation, the hydrazinolysis-nitrosation sequence should be applied to the reduced oligosaccharides released on cleavage with alkali. |
| Databáze: | OpenAIRE |
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