Characterization of chondroitin sulfate in stem cells derived from umbilical cord blood in rats
Autor: | Keiko Nakanishi, Kyohei Higashi, Toshihiko Toida, Masato Asai |
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Rok vydání: | 2021 |
Předmět: |
Embryology
Physiology Science Cell Culture Techniques Carbohydrates Disaccharides Umbilical Arteries Umbilical Cord Animal Cells Medicine and Health Sciences Animals Sulfation Cells Cultured Cell Proliferation Clinical Genetics Multidisciplinary Organic Compounds Sulfates Stem Cells Stem Cell Therapy Chondroitin Sulfates Organic Chemistry Chemical Compounds Chemical Reactions Biology and Life Sciences Cell Differentiation Cell Biology Arteries Fetal Blood Hematopoietic Stem Cells Rats Body Fluids Chemistry Blood Physical Sciences Cardiovascular Anatomy Medicine Blood Vessels Female Salts Anatomy Cellular Types Research Article Developmental Biology |
Zdroj: | PLoS ONE PLoS ONE, Vol 17, Iss 1 (2022) PLoS ONE, Vol 17, Iss 1, p e0262854 (2022) |
ISSN: | 1932-6203 |
Popis: | Chondroitin sulfate (CS) and its isomeric variant, dermatan sulfate (DS), are complex glycosaminoglycans (GAGs) which are ubiquitous components of the extracellular matrix in various tissues including the brain. CS and/or DS are known to bind to a variety of growth factors and regulate many cellular events such as proliferation and differentiation. Although the biological activities of CS and/or DS towards neural stem/progenitor cells (NSPCs) have been well investigated, the CS and/or DS of hematopoietic stem cells (HSCs) have not been fully characterized. Here, we analyzed GAGs on mononuclear cells of rat umbilical cord blood cells (UCB-MNCs). CS was detected in vascular intima and media of rat umbilical cord at embryonic day 19 (E19) by immunohistochemistry. The stem-cell-enriched-UCBCs (SCE-UCBCs), which were expanded from rat UCB-MNCs, expressed CS. CS chains are composed of repeating disaccharide units, which are classified into several types such as O-, A-, B-, C-, D-, and E-unit according to the number and positions of sulfation. A disaccharide composition analysis revealed that CS and/or DS were abundant in rat UCB-MNCs as well as in their expanded SCE-UCBCs, while the amount of heparan sulfate (HS) was less. The degree of sulfation of CS/DS was relatively low and the major component in UCB-MNCs and SCE-UCBCs was the A-unit. A colony-forming cell assay revealed that the percentage of colony-forming cells decreased in culture with CS degradation enzyme. The CS and/or DS of UCBCs may be involved in biological activities such as stem cell proliferation and/or differentiation. |
Databáze: | OpenAIRE |
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