Cytotoxic and apoptotic inducing activity of Amoora rohituka leaf extracts in human breast cancer cells
| Autor: | Neelam Atri, Santosh Kumar Singh, Anil Kumar Shukla, Akhileshwar Kumar Srivastava, Anil Kumar Singh, Rajesh Kumar Singh, Monika Singh, Anurag Mishra, Amit Ranjan |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0211 other engineering and technologies
Ethyl acetate Apoptosis 02 engineering and technology DNA laddering HPTLC 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Breast cancer 021105 building & construction Drug Discovery Petroleum ether Cell migration IC50 lcsh:Miscellaneous systems and treatments Traditional medicine biology biology.organism_classification lcsh:RZ409.7-999 Terpenoid 030205 complementary & alternative medicine Complementary and alternative medicine chemistry Phytochemical FTIR Original Research Article (Experimental) Amoora |
| Zdroj: | Journal of Ayurveda and Integrative Medicine, Vol 11, Iss 4, Pp 383-390 (2020) Journal of Ayurveda and Integrative Medicine |
| ISSN: | 0975-9476 |
| Popis: | Background Amoora rohituka is described in Ayurveda, an Indian traditional system of medicine for management of disorders of blood, diseases of eye, helminthiasis disease, ulcer, liver disorders and splenomegaly. However, the leaves were not reported to have anticancer properties till date. Objective This study was carried out to evaluate the cytotoxic potential of leaf extracts of Amoora rohituka. Materials and methods The leaves powder was macerated in petroleum ether, ethyl acetate and methanol and evaluated their anticancer activities in vitro. The phytochemical constituents of the active (ethyl acetate) extract were screened by FTIR analysis and phytochemical screening methods. Results The ethyl acetate extract (RLEA) showed the presence of alkaloids, flavonoids, steroids, tannins, saponins and terpenoids. The RLEA exhibited high cytotoxic effect against human breast cancer cells, MCF-7 (IC50 = 9.81 μg/mL) and induced apoptosis by altering nuclear morphology and DNA laddering. Wound healing assays explained the potency of extract to decrease the cell migration. Conclusion The extract of Amoora rohituka leaves exhibited anticancer activity with less toxicity and it could be used for development of alternative drugs in the treatment of human breast cancer. Graphical abstract Image 1 |
| Databáze: | OpenAIRE |
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