Thermal damage assessment of blood vessels in a hamster skin flap model by fluorescence measurement of a liposome-dye system
| Autor: | Jean-Marie Devoisselle, S. Soulié, Serge Mordon, Thomas Desmettre |
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| Rok vydání: | 1997 |
| Předmět: |
Indocyanine Green
Male Fluorescence-lifetime imaging microscopy Sonication Analytical chemistry Dermatology Fluorescence Surgical Flaps law.invention chemistry.chemical_compound law Cricetinae medicine Animals Irradiation Skin Liposome Drug Carriers Laser Coagulation Laser Fluoresceins medicine.anatomical_structure chemistry Liposomes Blood Vessels Surgery Indocyanine green Biomedical engineering Blood vessel |
| Zdroj: | Lasers in surgery and medicine. 20(2) |
| ISSN: | 0196-8092 |
| Popis: | Background and Objectives The present study was undertaken to evaluate the feasibility of thermal damage assessment of blood vessels by using laser-induced release of liposome-encapsulated dye. Study Design/Materials and Methods Experiments were performed in a hamster skin flap model. Laser irradiation was achieved with a 300 μm fiber connected to a 805 nm diode laser (power=0.8W, spot diameter=1.3 mm and pulse exposure time lasting from 1 to 6 s) after potentiation using a specific indocyanine green (ICG) formulation (water and oil emulsion). Liposomes-encapsulated carboxyfluorescein were prepared by the sonication procedure. Carboxyfluorescein (5,6-CF) was loaded at high concentration (100 mM) in order to quench its fluorescence. The measurements were performed after i.v. injection of DSPC liposomes (1.5 ml) and lasted 40 min. Fluorescence emission was measured with an ultra high sensitivity intensified camera. Results Three different shapes of fluorescent spots were identified depending on target (blood vessel or skin) and energy deposition in tissue: (i) intravascular fluorescence, (ii) transient low fluorescence circular spot, and (iii) persistent high intense fluorescence spot. These images are correlated with histological data. Conclusion Real-time fluorescence imaging seems to be a good tool to estimate in a non-invasive manner the thermal damage induced by a diode laser combined with ICG potentiation. Lasers Surg. Med. 20:131–141, 1997. © 1997 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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