The Tetracycline-Controlled Transactivator (Tet-On/Off) System in β-Cells Reduces Insulin Expression and Secretion in Mice
| Autor: | Khalil Bouyakdan, Cindy Baldwin, Scott A. Campbell, Maureen Gannon, Thierry Alquier, Vincent Poitout, Nathalie Jouvet, Jennifer L. Estall, Shannon E. Townsend |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Endocrinology Diabetes and Metabolism medicine.medical_treatment Transgene Cre recombinase Mice Transgenic Biology Mice Transactivation Insulin-Secreting Cells Internal Medicine medicine Animals Insulin Gene silencing Glucose homeostasis Transgenes Promoter Regions Genetic Cells Cultured Integrases Tetracycline Cell biology Mice Inbred C57BL Islet Studies Gene Expression Regulation Trans-Activators Female Ectopic expression Beta cell |
| Zdroj: | Diabetes |
| ISSN: | 1939-327X 0012-1797 |
| DOI: | 10.2337/db21-0147 |
| Popis: | Controllable genetic manipulation is an indispensable tool in research, greatly advancing our understanding of cell biology and physiology. However in β-cells, transgene silencing, low inducibility, ectopic expression, and off-targets effects are persistent challenges. In this study, we investigated whether an inducible Tetracycline (Tet)-Off system with β-cell–specific mouse insulin promoter (MIP)-itTA–driven expression of tetracycline operon (TetO)-CreJaw/J could circumvent previous issues of specificity and efficacy. Following assessment of tissue-specific gene recombination, β-cell architecture, in vitro and in vivo glucose-stimulated insulin secretion, and whole-body glucose homeostasis, we discovered that expression of any tetracycline-controlled transactivator (e.g., improved itTA, reverse rtTA, or tTA) in β-cells significantly reduced Insulin gene expression and decreased insulin content. This translated into lower pancreatic insulin levels and reduced insulin secretion in mice carrying any tTA transgene, independent of Cre recombinase expression or doxycycline exposure. Our study echoes ongoing challenges faced by fundamental researchers working with β-cells and highlights the need for consistent and comprehensive controls when using the tetracycline-controlled transactivator systems (Tet-On or Tet-Off) for genome editing. |
| Databáze: | OpenAIRE |
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