An anti-tumor role for cGMP-dependent protein kinase
| Autor: | Yali Hou, Elsie Wong, Robert G. Martindale, Vadivel Ganapathy, Naren Gupta, Patricia V. Schoenlein, Darren D. Browning |
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| Rok vydání: | 2005 |
| Předmět: |
Cancer Research
Transcription Genetic Down-Regulation Mice Nude Biology Adenocarcinoma Transfection Mice Nude mouse Genes Reporter Complementary DNA Cell Line Tumor Gene expression medicine Cyclic GMP-Dependent Protein Kinases Animals Humans Neoplasm Invasiveness RNA Messenger Protein kinase A Promoter Regions Genetic Mice Inbred BALB C biology.organism_classification Molecular biology Epithelium Blot Gene Expression Regulation Neoplastic medicine.anatomical_structure Oncology Cell culture Tissue Array Analysis cardiovascular system Colorectal Neoplasms cGMP-dependent protein kinase Neoplasm Transplantation |
| Zdroj: | Cancer letters. 240(1) |
| ISSN: | 0304-3835 |
| Popis: | This study compared Type-1 cGMP-dependent protein kinase (PKG) expression in normal and tumor tissues and examined PKG function in tumor growth. Studies with a cDNA array revealed that PKG expression was reduced in many tumors compared to respective normal tissue. This decrease in PKG expression was confirmed using quantitative RT-PCR and western blotting of matched colon specimens from normal epithelium and tumor tissue, and also in colon derived cell lines where luciferase reporter analysis revealed that the decreased expression occurred at the transcriptional level. Using SW620 colon carcinoma cells engineered for inducible expression of PKG1beta, it was found that exogenous PKG1beta lead to decreased tumor growth and invasiveness in nude mouse xenografts. |
| Databáze: | OpenAIRE |
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