Hypermethylation of RASSF1A in human and rhesus placentas
| Autor: | Ryan K. C. Yuen, Yu K. Tong, Tse N. Leung, Joanna H.M. Tong, Christy Ferlatte, Lisa Y.S. Chan, Ka F. To, Rossa W.K. Chiu, Y.M. Dennis Lo, Alisa S.W. Shum, Ivy H.N. Wong, John K.C. Chan, Wing Shan Lee, Kwok W. Lo, Cesar S.C. Wong, Tze K. Lau, Jessie Wai-Fong Yuen, Stephen S.C. Chim, Jingly F. Weier |
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| Rok vydání: | 2007 |
| Předmět: |
endocrine system
Tumor suppressor gene Placenta Bisulfite sequencing Molecular Sequence Data Gene Expression Biology Pathology and Forensic Medicine Epigenesis Genetic Mice Pregnancy Cell Line Tumor Gene expression medicine Biomarkers Tumor Animals Humans Genes Tumor Suppressor Epigenetics Amino Acid Sequence Promoter Regions Genetic reproductive and urinary physiology DNA Primers Sequence Homology Amino Acid Reverse Transcriptase Polymerase Chain Reaction Lasers Tumor Suppressor Proteins Methylation DNA Methylation Molecular biology Immunohistochemistry Macaca mulatta medicine.anatomical_structure Trichostatin A DNA methylation embryonic structures Female Microdissection medicine.drug Regular Articles |
| Zdroj: | The American journal of pathology. 170(3) |
| ISSN: | 0002-9440 |
| Popis: | The pseudomalignant nature of the placenta prompted us to search for tumor suppressor gene hypermethylation, a phenomenon widely reported in cancer, in the human placenta. Nine tumor suppressor genes were studied. Hypermethylation of the Ras association domain family 1 A (RASSF1A) gene was found in human placentas from all three trimesters of pregnancy but was absent in other fetal tissues. Hypermethylation of Rassf1 was similarly observed in placentas from the rhesus monkey but not the mouse. An inverse relationship between RASSF1A promoter methylation and gene expression was demonstrated by bisulfite sequencing of microdissected placental cells and immunohistochemical staining of placental tissue sections using an anti-RASSF1A antibody. Treatment of choriocarcinoma cell lines, JAR and JEG3, by 5-aza-2′-deoxycytidine and trichostatin A led to reduction in RASSF1A methylation but increased expression. These observations extend the analogy between the primate placenta and malignant tumors to the epigenetic level. |
| Databáze: | OpenAIRE |
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