Adrenergic Effect on Cytokine Release After Ex Vivo Healthy Volunteers' Whole Blood LPS Stimulation
| Autor: | George Baltopoulos, Pavlos Myrianthefs, Theodoros Katsoulas, Vasiliki Papandreou, Kyriaki Venetsanou, Nadia Kavrochorianou |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides medicine.medical_specialty medicine.medical_treatment Immunology Stimulation Norepinephrine (medication) Sepsis 03 medical and health sciences Norepinephrine 0302 clinical medicine Adrenergic Agents Internal medicine Dobutamine medicine Immunology and Allergy Humans Whole blood Cytokine Measurement Dose-Response Relationship Drug business.industry Interleukin-6 Tumor Necrosis Factor-alpha 030208 emergency & critical care medicine medicine.disease Healthy Volunteers 030104 developmental biology Endocrinology Cytokine Blood Cytokines business Ex vivo medicine.drug |
| Zdroj: | Inflammation. 39(3) |
| ISSN: | 1573-2576 |
| Popis: | Catecholamines are molecules with immunomodulatory properties in health and disease. Several studies showed the effect of catecholamines when administered to restore hemodynamic stability in septic patients. This study investigates the effect of norepinephrine and dobutamine on whole blood cytokine release after ex vivo lipopolysaccharide (LPS) stimulation. Whole blood collected from healthy individuals was stimulated with LPS, in the presence of norepinephrine or dobutamine at different concentrations, with or without metoprolol, a β1 receptor antagonist. Cytokine measurement was performed in isolated cell culture supernatants with ELISA. Results are expressed as mean ± SEM and compared with Mann-Whitney rank-sum test. Both norepinephrine and dobutamine significantly reduced TNF-α and IL-6 production after ex vivo LPS stimulation of whole blood in a dose-dependent manner, and this effect was partially reversed by the presence of metoprolol. Norepinephrine and dobutamine reduce the LPS-induced production of pro-inflammatory cytokines, thus possibly contributing to altered balance between the inflammatory and anti-inflammatory responses, which are vital for a successful host response to severe disease, shock, and sepsis. |
| Databáze: | OpenAIRE |
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