Effect of Adipose‐Derived Stem Cells on Head and Neck Squamous Cell Carcinoma
Autor: | Shayn M. Peirce, Paul D. Koors, Mark J. Jameson, Brian Langford, Michael I. Dougherty, Christine E. Lehman, Rolando E. Mendez, Daniel Gioeli, Deepa Danan |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Cell Survival Cell Culture Techniques Mice Nude Mice 03 medical and health sciences 0302 clinical medicine stomatognathic system In vivo Cell Line Tumor otorhinolaryngologic diseases medicine Animals Humans Clonogenic assay Cell Proliferation Squamous Cell Carcinoma of Head and Neck Cell growth business.industry Stem Cells Cancer hemic and immune systems medicine.disease Head and neck squamous-cell carcinoma eye diseases Disease Models Animal stomatognathic diseases 030104 developmental biology Adipose Tissue Otorhinolaryngology Cell culture 030220 oncology & carcinogenesis Cancer research Female Surgery Stem cell business Wound healing |
Zdroj: | Otolaryngology–Head and Neck Surgery. 158:882-888 |
ISSN: | 1097-6817 0194-5998 |
Popis: | Objective Patients with head and neck squamous cell carcinoma (HNSCC) have significant wound-healing difficulties. While adipose-derived stem cells (ASCs) facilitate wound healing, ASCs may accelerate recurrence when applied to a cancer field. This study evaluates the impact of ASCs on HNSCC cell lines in vitro and in vivo. Study Design In vitro experiments using HNSCC cell lines and in vivo mouse experiments. Setting Basic science laboratory. Subjects and Methods Impact of ASCs on in vitro proliferation, survival, and migration was assessed using 8 HNSCC cell lines. One cell line was used in a mouse orthotopic xenograft model to evaluate in vivo tumor growth in the presence and absence of ASCs. Results Addition of ASCs did not increase the number of HNSCC cells. In clonogenic assays to assess cell survival, addition of ASCs increased colony formation only in SCC9 cells (maximal effect 2.3-fold, P < .02) but not in other HNSCC cell lines. In scratch assays to assess migration, fluorescently tagged ASCs did not migrate appreciably and did not increase the rate of wound closure in HNSCC cell lines. Addition of ASCs to HNSCC xenografts did not increase tumor growth. Conclusion Using multiple in vitro and in vivo approaches, ASCs did not significantly stimulate HNSCC cell proliferation or migration and increased survival in only a single cell line. These findings preliminarily suggest that the use of ASCs may be safe in the setting of HNSCC but that further investigation on the therapeutic use of ASCs in the setting of HNSCC is needed. |
Databáze: | OpenAIRE |
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