Automatic mass spectrometry method development for drug discovery: application in metabolic stability assays
| Autor: | Candace Black-Schaefer, Yau Yi Lau, Peter J. Dandliker, Linda E. Chovan |
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| Rok vydání: | 2004 |
| Předmět: |
Chromatography
Drug discovery Chemistry Organic Chemistry Drug Evaluation Preclinical Metabolic stability In vitro incubation Tandem mass spectrometry Software package Mass spectrometry Method development Sensitivity and Specificity Mass Spectrometry Analytical Chemistry Automation Robotic systems Drug Stability Pharmaceutical Preparations Microsomes Spectroscopy |
| Zdroj: | Rapid communications in mass spectrometry : RCM. 18(24) |
| ISSN: | 0951-4198 |
| Popis: | High-throughput metabolic screening has been requested routinely to keep pace with high-throughput organic synthesis. Liquid chromatography/tandem mass spectrometry (LC/MS/MS) with a fast gradient has become the method of choice for the task due to its sensitivity and selectivity. We have developed an automated system that consists of a robotic system for in vitro incubation and a commercially available software package for automatic MS/MS method development. A short, generic LC gradient and MS conditions that are applicable to most compounds have been developed to minimize the method development time and data analysis. This system has been used to support a number of in vitro screening assays in early drug discovery phase including microsomal stability and protein binding. |
| Databáze: | OpenAIRE |
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