Establishment and characterization of a prostate cancer cell line from a prostatectomy specimen for the study of cellular interaction

Autor: Peizhen Hu, Sen Pathak, Haiyen E. Zhau, Gina C.Y. Chu, Xudong Wang, Leland W.K. Chung, Jason Boyang Wu, Asha S. Multani, Ruoxiang Wang
Rok vydání: 2019
Předmět:
Zdroj: Int J Cancer
ISSN: 1097-0215
0020-7136
DOI: 10.1002/ijc.32370
Popis: Though human prostate cancer (PCa) heterogeneity can best be studied using multiple cell types isolated from clinical specimens, the difficulty of establishing cell lines from clinical tumors has hampered this approach. In this proof-of-concept study we established a human PCa cell line from a prostatectomy surgical specimen without the need for retroviral transduction. In a previous report, we characterized the stromal cells derived from PCa specimens. Here, we characterized the epithelial cells isolated from the same tumors. Compared to the ease of establishing prostate stromal cell lines, prostatic epithelial cell lines are challenging. From three matched pairs of normal and tumor tissues, we established one new PCa cell line, HPE-15. We confirmed the origin of HPE-15 cells by short tandem repeat (STR) microsatellite polymorphism analysis. HPE-15 cells are androgen-insensitive and express marginal androgen receptor (AR), prostate specific antigen (PSA), and prostate specific membrane antigen (PSMA) proteins. HPE-15 expresses luminal epithelial markers of E-cadherin and cytokeratin 18, basal cell markers of cytokeratin 5 and p63, and neuroendocrine marker of chromogranin A (CgA). Interestingly, HPE-15 cells showed no tumorigenicity in different strains of immune-deficient mcie, but can become tumorigenic through interaction with aggressive cancer cell types. HPE-15 cells can thus serve as an experimental model for the study of PCa progression, metastasis, and tumor cell dormancy.
Databáze: OpenAIRE
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