The mechanism by which adenosine decreases gluconeogenesis from lactate in isolated rat hepatocytes
| Autor: | S. Claeyssens, H.A. Buc, F Matray, A Lavoinne, M. Pinosa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1987 |
| Předmět: |
Pyruvate decarboxylation
medicine.medical_specialty Adenosine Adenosine kinase Ketone Bodies In Vitro Techniques Biochemistry Phosphates chemistry.chemical_compound Adenosine deaminase Adenine nucleotide Internal medicine Pyruvic Acid medicine Cyclic AMP Animals Lactic Acid Pyruvates Molecular Biology Alanine biology Adenine Nucleotides Gluconeogenesis Cell Biology Rats Endocrinology chemistry Liver biology.protein Deoxycoformycin Lactates Pyruvic acid medicine.drug Research Article |
| Popis: | Incubation of hepatocytes from 24 h-starved rats in the presence of 0.5 mM-adenosine decreased gluconeogenesis from lactate, but not from alanine. The inhibition of gluconeogenesis was associated with a stimulation of ketone-body production and an inhibition of pyruvate oxidation. These metabolic changes were suppressed in the presence of iodotubercidin (an inhibitor of adenosine kinase), but were reinforced in the presence of deoxycoformycin (an inhibitor of adenosine deaminase); 2-chloroadenosine induced no change in gluconeogenesis from lactate. These data indicate that the inhibition of gluconeogenesis by adenosine probably results from its conversion into adenine nucleotides. In the presence of lactate or pyruvate, but not with alanine or asparagine, this conversion resulted in a decrease in the [ATP]/[ADP] ratio in both mitochondrial and cytosolic compartments. Adenosine decreased the Pi concentration with all gluconeogenic substrates. |
| Databáze: | OpenAIRE |
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