Free radical production in aortic rings from rats fed a fish oil-rich diet
| Autor: | Catalina Caballero, M. Teresa Mitjavila, Diego López, P. Puig-Parellada, Juan Sebastián Vera Sánchez |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Nitroprusside Vascular smooth muscle Free Radicals Physiology Vasodilator Agents Radical Administration Oral In Vitro Techniques Nitric oxide Rats Sprague-Dawley Phenylephrine chemistry.chemical_compound Fish Oils Dietary Fats Unsaturated Superoxides Physiology (medical) medicine.artery medicine Animals Vasoconstrictor Agents Food science Aorta Food Formulated chemistry.chemical_classification Degree of unsaturation Nitrates Dose-Response Relationship Drug Superoxide Dismutase Body Weight Fish oil Acetylcholine Rats Vasodilation chemistry Biochemistry Prostaglandin-Endoperoxide Synthases Vasoconstriction Cardiology and Cardiovascular Medicine Corn oil Polyunsaturated fatty acid |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 280:H2929-H2935 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.2001.280.6.h2929 |
| Popis: | To study the effect of the degree of unsaturation of dietary fatty acids on the production of free radicals and on the vascular smooth muscle tone in rings of the aorta, Sprague-Dawley rats were fed a semipurified diet containing 5% lipids from either corn oil (CO) or menhaden oil (MO) for 8 wk. The MO diet did not change the basal or NADPH-dependent superoxide anion (O[Formula: see text]·) release. There were no significant differences in phenylephrine-induced contractions between the two groups in intact rings. However, these contractions increased in endothelium-intact aortic rings from the MO group after addition of the nitric oxide (·NO) synthase inhibitor N G-nitro-l-arginine and in endothelium-denuded rings, both indicating a greater endothelial basal ·NO production in rats fed with the MO diet. Endothelium-dependent relaxations in response to acetylcholine were more prominent in rings from the MO group. These differences were not due to an increased smooth muscle response to ·NO, because relaxations were the same using an exogenous ·NO donor. Our results indicate that dietary MO did not modify O[Formula: see text]· release by the vessel wall or relaxation due to the cyclooxygenase pathway, but it potentiated endothelial production of ·NO. |
| Databáze: | OpenAIRE |
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