Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin

Autor: Haruki Kitazawa, Akm Humayun Kober, Asuka Tada, Kazutoyo Yoda, Fang He, Hisashi Aso, Kenji Miyazawa, Julio Villena, Aminul Islam, Manami Igata, Hideki Takahashi, Kohtaro Fukuyama, Leonardo Albarracin, Michihiro Takagi, Wakako Ikeda-Ohtsubo
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Swine
Stimulation
Transcriptome
lcsh:Chemistry
chemistry.chemical_compound
porcine mature adipocyte (pma)
Adipocyte
CEBPB
Adipocytes
Gene Regulatory Networks
lcsh:QH301-705.5
Spectroscopy
Cells
Cultured
Chemistry
SEROTONIN
Cell Differentiation
purl.org/becyt/ford/3.1 [https]
General Medicine
Computer Science Applications
Cell biology
serotonin
PORCINE INTRAMUSCULAR PREADIPOCYTE (PIP)
Adipogenesis
purl.org/becyt/ford/3 [https]
Female
Lipid digestion
serotonin receptor
PORCINE MATURE ADIPOCYTE (PMA)
Catalysis
Article
adipogenesis
Inorganic Chemistry
porcine intramuscular preadipocyte (pip)
TNF-Α
Animals
TRANSCRIPTOME
Physical and Theoretical Chemistry
Muscle
Skeletal
Molecular Biology
Cell chemotaxis
Dose-Response Relationship
Drug
tnf-α
Tumor Necrosis Factor-alpha
Gene Expression Profiling
Organic Chemistry
Lipid metabolism
Lipid Metabolism
ADIPOGENESIS
SEROTONIN RECEPTOR
Gene Expression Regulation
lcsh:Biology (General)
lcsh:QD1-999
transcriptome
Zdroj: International Journal of Molecular Sciences, Vol 21, Iss 2, p 638 (2020)
International Journal of Molecular Sciences
Volume 21
Issue 2
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
ISSN: 1422-0067
Popis: Adipocytes are dynamic cells that have critical functions to maintain body energy homeostasis. Adipocyte physiology is affected by the adipogenic differentiation, cell program, as well as by the exogenous stimulation of biochemical factors, such as serotonin and TNF-&alpha
In this work, we investigated the global transcriptome modifications when porcine intramuscular preadipocyte (PIP) was differentiated into porcine mature adipocyte (pMA). Moreover, we studied transcriptome changes in pMA after stimulation with serotonin or TNF-&alpha
by using a microarray approach. Transcriptome analysis revealed that the expression of 270, 261, and 249 genes were modified after differentiation, or after serotonin and TNF-&alpha
stimulation, respectively. Expression changes in APP, HNF4A, ESR1, EGR1, SRC, HNF1A, FN1, ALB, STAT3, CBL, CEBPB, AR, FOS, CFTR, PAN2, PTPN6, VDR, PPARG, STAT5A and NCOA3 genes which are enriched in the &lsquo
PPAR signaling&rsquo
and &lsquo
insulin resistance&rsquo
pathways were found in adipocytes during the differentiation process. Dose-dependent serotonin stimulation resulted in a decreased fat accumulation in pMAs. Serotonin-induced differentially expressed genes in pMAs were found to be involved in the significant enrichment of &prime
GPCR ligand-binding&prime
&lsquo
cell chemotaxis&rsquo
blood coagulation and complement&rsquo
metabolism of lipid and lipoproteins&rsquo
regulation of lipid metabolism by PPARA&rsquo
lipid digestion, mobilization and transport&rsquo
pathways. TNF-&alpha
stimulation also resulted in transcriptome modifications linked with proinflammatory responses in the pMA of intramuscular origin. Our results provide a landscape of transcriptome modifications and their linked-biological pathways in response to adipogenesis, and exogenous stimulation of serotonin- and TNF-&alpha
to the pMA of intramuscular origin.
Databáze: OpenAIRE
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