Membrane fluidity of Toxoplasma gondii: a fluorescence polarization study
| Autor: | Geneviève Mauras, Anne Tricaud, Jocelyne Hodbert, A Girault, Yves Gallois, Françoise Foussard, Claude Motta |
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| Rok vydání: | 1988 |
| Předmět: |
Membrane Fluidity
Phospholipid Fluorescence Polarization chemistry.chemical_compound Phosphatidylcholine parasitic diseases Membrane fluidity Animals Phospholipids Unsaturated fatty acid biology Cell Membrane Fatty Acids Toxoplasma gondii Cell Biology General Medicine biology.organism_classification Lipids Fluorescence Kinetics Cholesterol Biochemistry chemistry lipids (amino acids peptides and proteins) Sphingomyelin Diphenylhexatriene Toxoplasma Fluorescence anisotropy |
| Zdroj: | Biology of the Cell. 62:11-15 |
| ISSN: | 0248-4900 |
| DOI: | 10.1111/j.1768-322x.1988.tb00701.x |
| Popis: | Toxoplasma gondii membrane fluidity was investigated by fluorescence polarization. We used 1,6-diphenyl 1,3,5-hexatriene (DPH) as a fluorescent hydrophobic probe. Fluorescence anisotropy (r) and degree of order (s) showed high fluidity properties. Chemical analysis was performed on this parasite. We found a low cholesterol/phospholipid ratio, many unsaturated fatty acids chains, and high phosphatidylcholine and low sphingomyelin amounts. These results were in good agreement with the observed high fluidity. This may be related to the great adaptability of Toxoplasma gondii in infesting a wide variety of host cells. |
| Databáze: | OpenAIRE |
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