Overexpression of activating transcription factor 3 exerts suppressive effects in HepG2 cells
| Autor: | Jiasi Li, Aimin Huang, Haili Cheng, Xiaoyan Li, Shengbing Zang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research lentiviral vectors of overexpression Cell Activating transcription factor Apoptosis Biochemistry Flow cytometry 03 medical and health sciences 0302 clinical medicine Cell Movement Cell Line Tumor Genetics medicine Humans cytological mechanism Molecular Biology Cell Proliferation Activating Transcription Factor 3 Oncogene medicine.diagnostic_test Cell growth Chemistry Cell Cycle Liver Neoplasms Hep G2 Cells Articles Cell cycle Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer cell Cancer research Molecular Medicine |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 |
| Popis: | The present study observed and compared the biological behaviour of HepG2 cells prior and subsequent to the overexpression of activating transcription factor 3 (ATF3). Experiments investigating the cytological function by which ATF3 affects liver cancer cells were also performed. MTT, Transwell and flow cytometry assays were used to observe and detect the biological behaviour of HepG2 cells with and without lentivirus (LV)-ATF3-enhanced green fluorescent protein (EGFP) infection. The effects of ATF3 overexpression on cell proliferation, migration, apoptosis and cell cycle progression were evaluated. The LV-ATF3-EGFP overexpression vector was successfully constructed, and the HepG2 cells were successfully infected with the vector. Following ATF3 overexpression, cell proliferation was decreased, the rate of cell apoptosis was accelerated and cell cycle progression was slowed (P0.05), although there was a trend towards a gradual decrease. In conclusion, ATF3 exerted suppressive effects in HepG2 cells, potentially by inhibiting cancer cell growth, accelerating cell apoptosis, and blocking cell cycle progression. Intervention targeting ATF3 expression may represent a novel approach for the prevention and treatment of human liver cancer. |
| Databáze: | OpenAIRE |
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