Avian CD154 enhances humoral and cellular immune responses induced by an adenovirus vector-based vaccine in chickens
| Autor: | Lester Suárez Amarán, Natalie C. Parra, Julia Noda Gómez, Alaín González Pose, Julio César Águila Benites, Armando Vega Redondo, Silvia Gómez-Puerta, Jorge Roberto Toledo Alonso, Oliberto Sánchez Ramos |
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| Rok vydání: | 2010 |
| Předmět: |
Cellular immunity
medicine.medical_treatment Recombinant Fusion Proteins Immunology CD40 Ligand Genetic Vectors chemical and pharmacologic phenomena Biology Antibodies Viral Microbiology Viral vector Adenoviridae Cell Line Interferon-gamma Mice Immune system Antigen Gene Order medicine Immunology and Allergy Animals Immunity Cellular General Veterinary Immunogenicity Viral Vaccines General Medicine Virology Immunity Humoral Vaccination Infectious Diseases Humoral immunity Leukocytes Mononuclear Adjuvant Chickens |
| Zdroj: | Comparative immunology, microbiology and infectious diseases. 34(3) |
| ISSN: | 1878-1667 |
| Popis: | Recombinant adenoviral vectors have emerged as an attractive system for veterinary vaccines development. However, for poultry vaccination a very important criterion for an ideal vaccine is its low cost. The objective of this study was to test the ability of chicken CD154 to enhance the immunogenicity of an adenoviral vector-based vaccine against avian influenza virus in order to reduce the amount of antigen required to induce an effective immune response in avian. Chickens were vaccinated with three different doses of adenoviral vectors encoding either HA (AdHA), or HA fused to extracellular domain chicken's CD154 (AdHACD). Hemagglutination inhibition (HI) assay and relative quantification of IFN-γ showed that the adenoviral vector encoding for the chimeric antigen is able to elicit an improved humoral and cellular immune response, which demonstrated that CD154 can be used as a molecular adjuvant allowing to reduce in about 50-fold the amount of adenoviral vector vaccine required to induce an effective immune response. |
| Databáze: | OpenAIRE |
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