Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1
| Autor: | Dominique Le Ray, Rémy Bosselut, J Ghysdael, Marie Geneviève Mattei, Françoise Moreau-Gachelin, Armand Tavitian |
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| Rok vydání: | 1992 |
| Předmět: |
animal structures
Transcription Genetic animal diseases Molecular Sequence Data Retroviridae Proteins Oncogenic Sequence Homology Biology DNA-binding protein Homology (biology) Cell Line Mice Transcription (biology) Complementary DNA Tumor Cells Cultured Animals Humans Amino Acid Sequence Cloning Molecular Molecular Biology Transcription factor Peptide sequence Gene Base Sequence Protein primary structure Chromosome Mapping DNA Cell Biology biochemical phenomena metabolism and nutrition Blotting Northern bacterial infections and mycoses Burkitt Lymphoma Molecular biology DNA-Binding Proteins Gene Expression Regulation bacteria Chromosomes Human Pair 19 Protein Binding Transcription Factors Research Article |
| Zdroj: | Molecular and Cellular Biology. 12:4297-4304 |
| ISSN: | 1098-5549 0270-7306 |
| DOI: | 10.1128/mcb.12.10.4297 |
| Popis: | We have cloned a human cDNA from a new gene, spi-B, on the basis of its homology with the DNA-binding domain of the Spi-1/PU.1 putative oncogene product. spi-B codes for a protein of 262 amino acids presenting 43% overall identity with Spi-1. Its highly basic carboxy-terminal region exhibits 34% sequence identity with the DNA-binding domain of the Ets-1 protein. We showed that the Spi-B protein is able to bind the purine-rich sequence (PU box) recognized by Spi-1/PU.1 and to activate transcription of a reporter plasmid containing PU boxes. Chromosome in situ hybridization allowed us to map spi-B to the 19q13.3-19q13.4 region of the human genome. spi-B, like spi-1, was found to be expressed in various murine and human hematopoietic cell lines except T lymphoid cell lines. |
| Databáze: | OpenAIRE |
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