Aedes aegypti:Induced Antibacterial Proteins Reduce the Establishment and Development ofBrugia malayi
| Autor: | Michael T. Ferdig, Philippe Bulet, Carl Lowenberger, Jules A. Hoffmann, Shirin Khalili, Bruce M. Christensen |
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| Rok vydání: | 1996 |
| Předmět: |
Brugia pahangi
Transcription Genetic Fat Body Molecular Sequence Data Immunology Aedes aegypti Brugia malayi Defensins Immune system Aedes Immunity parasitic diseases Escherichia coli Animals Microfilariae Defensin Antibacterial agent Infectivity Analysis of Variance Base Sequence biology fungi Blood Proteins DNA General Medicine Blotting Northern biology.organism_classification Virology Anti-Bacterial Agents Micrococcus luteus Culicidae Infectious Diseases RNA Parasitology Gerbillinae |
| Zdroj: | Experimental Parasitology. 83:191-201 |
| ISSN: | 0014-4894 |
| Popis: | The effect of host immune activation on the development of Brugia malayi in one susceptible and four refractory strains of Aedes aegypti and in Armigeres subalbatus was assessed. A. aegypti that were immune activated by the injection of saline or bacteria 24 hr before feeding on a B. malayi-infected gerbil had significantly reduced prevalences and mean intensities of infection from those of naive controls when exposed to bloodmeals with low (105 mf/20 microliters) and medium (160 mf/20 microliters) microfilaremias. At a higher microfilaremia (237 mf/20 microliters) there were no significant differences in mean intensities, suggesting that the number of parasites ingested may affect the host's ability to mount an effective defense response. Because the major immune proteins in A. aegypti are defensins, we did Northern analyses of fat body RNA 8 hr after immune activation or bloodfeeding. All mosquitoes demonstrated rapid transcriptional activity for defensins following immune activation by intrathoracic inoculation with either saline or bacteria. However, no strain of A. aegypti, susceptible or refractory to B. malayi, nor Ar. subalbatus produced defensin transcripts after bloodfeeding on an uninfected or a B. malayi-infected gerbil. These data suggest that inducible immune proteins of mosquitoes can reduce the prevalence and mean intensity of infections with ingested parasites, but these proteins are not expressed routinely after parasite ingestion and midgut penetration and probably do not contribute to existing refractory mechanisms. Immune proteins such as defensins, however, represent potential candidates to genetically engineer mosquitoes for resistance to filarial worms. |
| Databáze: | OpenAIRE |
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