Qiangji Jianli Decoction Alleviates Hydrogen Peroxide-Induced Mitochondrial Dysfunction via Regulating Mitochondrial Dynamics and Biogenesis in L6 Myoblasts
Autor: | Lingling Ke, Qun Du, Yanwu Li, Qing Li, Aidong Ji, Lanqi Li, Jian Liang, Wei Jiao, Yafang Song, Jinqiu Li, Jingwei Song, Huafeng Pan, Zhiwei Chen |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
FIS1
Aging Article Subject Mitochondrion medicine.disease_cause Biochemistry DNA Mitochondrial Mitochondrial Dynamics Superoxide dismutase Myoblasts medicine MFN1 Animals Humans NRF1 chemistry.chemical_classification Reactive oxygen species Organelle Biogenesis QH573-671 biology Cell Biology General Medicine Hydrogen Peroxide TFAM Cell biology Neoplasm Proteins Rats chemistry Antigens Surface biology.protein Cytology Oxidative stress Drugs Chinese Herbal Research Article |
Zdroj: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2021 (2021) |
ISSN: | 1942-0994 1942-0900 |
Popis: | Oxidative stress can cause the excessive generation of reactive oxygen species (ROS) and has various adverse effects on muscular mitochondria. Qiangji Jianli decoction (QJJLD) is an effective traditional Chinese medicine (TCM) that is widely applied to improve muscle weakness, and it has active constituents that prevent mitochondrial dysfunction. To investigate the protective mechanism of QJJLD against hydrogen peroxide- (H2O2-) mediated mitochondrial dysfunction in L6 myoblasts. Cell viability was determined with MTT assay. Mitochondrial ultrastructure was detected by transmission electron microscope (TEM). ROS and mitochondrial membrane potential (MMP) were analyzed by fluorescence microscope and flow cytometry. The superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, and malondialdehyde (MDA) level were determined by WST-1, TBA, and DTNB methods, respectively. The mRNA and protein levels were measured by quantitative real-time PCR (qRT-PCR) and Western blot. The cell viability was decreased, and the cellular ROS level was increased when L6 myoblasts were exposed to H2O2. After treatment with QJJLD-containing serum, the SOD and GSH-Px activities were increased. MDA level was decreased concurrently. ROS level was decreased while respiratory chain complex activity and ATP content were increased in L6 myoblasts. MMP loss was attenuated. Mitochondrial ultrastructure was also improved. Simultaneously, the protein expressions of p-AMPK, PGC-1α, NRF1, and TFAM were upregulated. The mRNA and protein expressions of Mfn1/2 and Opa1 were also upregulated while Drp1 and Fis1 were downregulated. These results suggest that QJJLD may alleviate mitochondrial dysfunction through the regulation of mitochondrial dynamics and biogenesis, the inhibition of ROS generation, and the promotion of mitochondrial energy metabolism. |
Databáze: | OpenAIRE |
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