Specific diagnosis of progressive multifocal leukoencephalopathy by polymerase chain reaction
| Autor: | Gerhard Hunsmann, Klaus Felgenhauer, B. Ruf, Eva Schielke, Wolfgang Lüke, W. Lüer, Thomas Weber, Stephan A. Frye, Rodney W. Turner, J. Haas, Hans-Dieter Pohle |
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| Rok vydání: | 1994 |
| Předmět: |
Molecular Sequence Data
JC virus HIV Infections Biology medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Virus law.invention 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid law medicine Immunology and Allergy Humans 030212 general & internal medicine Polymerase chain reaction Slow virus Base Sequence Progressive multifocal leukoencephalopathy Leukoencephalopathy Progressive Multifocal virus diseases medicine.disease Virology 3. Good health Infectious Diseases DNA Viral Viral disease Primer (molecular biology) Polyomavirus 030217 neurology & neurosurgery |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0022-1899 |
| Popis: | Using polymerase chain reaction (PCR), 34 cerebrospinal fluid (CSF) samples from 28 patients with progressive multifocal leukoencephalopathy (PML) were analyzed. As controls, 116 samples were evaluated from 82 human immunodeficiency virus type 1 (HIV-1)-infected patients and 1 HIV-1-negative patient. Of the HIV-1-positive patients, 23 had cerebral toxoplasmosis, 10 had HIV leukoencephalopathy, and 49 had other neurologic complications. Detection of JC virus (JCV) DNA in CSF was increased 10-fold by the addition of carrier DNA before phenol-chloroform-isoamyl alcohol extraction. The primer pair JC 26/29, from the VP1/large T region, had a limit of detection of 10(5) JCV DNA molecules/100 microL. The primer pair JC 36/39, located in the large T gene region, had a 100-fold lower limit of detection. With JC 26/29, the sensitivity was 43% (12/28) and specificity was 100%. Using JC 36/39, sensitivity increased to 82% (23/28), and false-positive results were not observed. Diagnosis of PML is greatly aided by PCR analysis of CSF. |
| Databáze: | OpenAIRE |
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