IFN-gamma-induced protein 10 is a novel biomarker of rhinovirus-induced asthma exacerbations
| Autor: | Peter G. Gibson, Sebastian L. Johnston, Stephen T. Holgate, Ammarin Thakkinstian, John Attia, Peter A. B. Wark, Giovanni Zummo, Donna E. Davies, Fabio Bucchieri, Lynnsey M. Hamilton, Joanna Mimica |
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| Přispěvatelé: | WARK, PA, BUCCHIERI, F, JOHNSTON, SL, GIBSON, PG, HAMILTON, L, MIMICA, J, ZUMMO, G, HOLGATE, ST, ATTIA, J, THAKKINSTIAN, A, DAVIES, DE |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Rhinovirus
Exacerbation NF-κB Nuclear factor κB Anti-Inflammatory Agents airway inflammation medicine.disease_cause Dexamethasone Immunology and Allergy Chemokine CCL5 Lung RV-16 Rhinovirus 16 Cells Cultured LR Likelihood ratio Respiratory disease Middle Aged Flow Cytometry medicine.anatomical_structure Biomarker (medicine) medicine.symptom Chemokines CXC medicine.drug Adult Adolescent Immunology Inflammation IFN gamma Article medicine Humans Aged Asthma Picornaviridae Infections Interleukin-6 Tumor Necrosis Factor-alpha business.industry Interleukin-8 BEC Bronchial epithelial cell Epithelial Cells TCID50 Tissue culture infectious dose 50% medicine.disease respiratory tract diseases Chemokine CXCL10 IP-10 IFN-γ–induced protein 10 business Biomarkers Respiratory tract |
| Zdroj: | The Journal of Allergy and Clinical Immunology |
| Popis: | BACKGROUND: Rhinovirus-induced acute asthma is the most frequent trigger for asthma exacerbations. OBJECTIVE: We assessed which inflammatory mediators were released from bronchial epithelial cells (BECs) after infection with rhinovirus and then determined whether they were also present in subjects with acute virus-induced asthma, with the aim to identify a biomarker or biomarkers for acute virus-induced asthma. METHODS: BECs were obtained from bronchial brushings of steroid-naive asthmatic subjects and healthy nonatopic control subjects. Cells were infected with rhinovirus 16. Inflammatory mediators were measured by means of flow cytometry with a cytometric bead array. Subjects with acute asthma and virus infection were recruited; they were characterized clinically by using lung function tests and had blood taken to measure the inflammatory mediators identified as important by the BEC experiments. RESULTS: IFN-gamma-induced protein 10 (IP-10) and RANTES were released in the greatest quantities, followed by IL-6, IL-8, and TNF-alpha. Dexamethasone treatment of BECs only partially suppressed IP-10 and TNF-alpha but was more effective at suppressing RANTES, IL-6, and IL-8. In acute clinical asthma serum IP-10 levels were increased to a greater extent in those with acute virus-induced asthma (median of 604 pg/mL compared with 167 pg/mL in those with non-virus-induced acute asthma, P < .01). Increased serum IP-10 levels were predictive of virus-induced asthma (odds ratio, 44.3 [95% CI, 3.9-100.3]). Increased serum IP-10 levels were strongly associated with more severe airflow obstruction (r = -0.8; P < .01). CONCLUSIONS: IP-10 release is specific to acute virus-induced asthma. CLINICAL IMPLICATIONS: Measurement of serum IP-10 could be used to predict a viral trigger to acute asthma. |
| Databáze: | OpenAIRE |
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