The effect of adrenalectomy on Fos expression in vasopressinergic and oxytocinergic neurons in response to stress in the rat

Autor:	Lucila Leico Kagohara Elias, M. de Castro, Carla Maria Ramos Germano, M. T. C. Laguna-Abreu, José Antunes-Rodrigues, Lisandra Oliveira Margatho
Rok vydání:	2007
Předmět:	Male endocrine system Vasopressin medicine.medical_specialty Vasopressins Physiology medicine.medical_treatment Oxytocin Inhibitory postsynaptic potential Behavioral Neuroscience Basal (phylogenetics) Parvocellular cell Internal medicine Adrenal Glands medicine Animals Rats Wistar Neurons Behavior Animal Endocrine and Autonomic Systems Chemistry Adrenalectomy Immunohistochemistry Rats Perfusion Psychiatry and Mental health Neuropsychology and Physiological Psychology Endocrinology nervous system Excitatory postsynaptic potential Proto-Oncogene Proteins c-fos Stress Psychological hormones hormone substitutes and hormone antagonists Glucocorticoid Paraventricular Hypothalamic Nucleus medicine.drug
Zdroj:	Stress. 10:332-341
ISSN:	1607-8888 1025-3890
Popis:	This study evaluated the responses of vasopressin (AVP) and oxytocin (OT) neurons to alterations in hypothalamo-pituitary axis activity by adrenalectomy (ADX) or after restraint stress compared with basal conditions. Wistar male rats were perfuse-fixed by cardiac perfusion under anesthesia 3 h, 1, 3 and 14 days after ADX or Sham surgery. Coronal hypothalamic sections were used for evaluation of Fos, AVP and OT expression by immunohistochemistry. Under basal conditions and after stress, Fos-AVP double labeling showed no difference in the magnocellular subdivisions of the paraventricular nuclei (PVN) or in the supraoptic nuclei (SON), suggesting that the magnocellular AVP system is unlikely to contribute to ACTH secretion after restraint in both Sham and ADX rats. Fos-AVP double labeling in the parvocellular medial paraventricular nucleus (PaMP) in ADX groups was increased after 3 h in basal conditions, and in all periods after restraint stress. There were no differences between Sham and ADX groups in Fos-OT double labeling in any subdivision of the PVN; however, in the SON, the number of Fos-OT double labeled cells was increased at all time-points after stress in the ADX group. Fos expression was increased in the PaMP after 3 h and after restraint stress in the Sham and ADX groups, especially in the ADX group. In conclusion, Fos expression in different cell populations of the PVN can be differentially regulated by short- and long-term absence of glucocorticoid negative feedback and also by stress-related excitatory and/or inhibitory neural inputs. The Fos-AVP double labeling findings in the PaMP also indicate a minor participation of these vasopressinergic neurons in the regulation of the HPA axis after ADX.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83f2eda1ba9a4895af8de77e85b7dace https://doi.org/10.1080/10253890701287614 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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