Airway microbiota signals anabolic and catabolic remodeling in the transplanted lung

Autor: Stéphane Mouraux, Eric Bernasconi, Céline Pattaroni, Angela Koutsokera, John-David Aubert, Johanna Claustre, Christophe Pison, Pierre-Joseph Royer, Antoine Magnan, Romain Kessler, Christian Benden, Paola M. Soccal, Benjamin J. Marsland, Laurent P. Nicod, J. Jougon, J.-F. Velly, H. Rozé, E. Blanchard, C. Dromer, M. Antoine, M. Cappello, M. Ruiz, Y. Sokolow, F. Vanden Eynden, G. Van Nooten, L. Barvais, J. Berré, S. Brimioulle, D. De Backer, J. Créteur, E. Engelman, I. Huybrechts, B. Ickx, T.J.C. Preiser, T. Tuna, L. Van Obberghe, N. Vancutsem, J.-L. Vincent, P. De Vuyst, I. Etienne, F. Féry, F. Jacobs, C. Knoop, J.L. Vachiéry, P. Van den Borne, I. Wellemans, G. Amand, L. Collignon, M. Giroux, D. Angelescu, O. Chavanon, R. Hacini, A. Pirvu, P. Porcu, P. Albaladejo, C. Allègre, A. Bataillard, D. Bedague, E. Briot, M. Casez-Brasseur, D. Colas, G. Dessertaine, M. Durand, G. Francony, A. Hebrard, M.R. Marino, B. Oummahan, D. Protar, D. Rehm, S. Robin, M. Rossi-Blancher, C. Augier, P. Bedouch, A. Boignard, H. Bouvaist, A. Briault, B. Camara, J. Claustre, S. Chanoine, M. Dubuc, S. Quétant, J. Maurizi, P. Pavèse, C. Pison, C. Saint-Raymond, N. Wion, C. Chérion, R. Grima, O. Jegaden, J.-M. Maury, F. Tronc, C. Flamens, S. Paulus, J.-F. Mornex, F. Philit, A. Senechal, J.-C. Glérant, S. Turquier, D. Gamondes, L. Chalabresse, F. Thivolet-Bejui, C. Barnel, C. Dubois, A. Tiberghien, F. Le Pimpec-Barthes, A. Bel, P. Mordant, P. Achouh, V. Boussaud, R. Guillemain, D. Méléard, M.O. Bricourt, B. Cholley, V. Pezella, G. Brioude, X.B. D'Journo, C. Doddoli, P. Thomas, D. Trousse, S. Dizier, M. Leone, L. Papazian, F. Bregeon, A. Basire, B. Coltey, N. Dufeu, H. Dutau, S. Garcia, J.Y. Gaubert, C. Gomez, S. Laroumagne, A. Nieves, L.C. Picard, M. Reynaud-Gaubert, V. Secq, G. Mouton, O. Baron, P. Lacoste, C. Perigaud, J.C. Roussel, I. Danner, A. Haloun, A. Magnan, A. Tissot, T. Lepoivre, M. Treilhaud, K. Botturi-Cavaillès, S. Brouard, R. Danger, J. Loy, M. Morisset, M. Pain, S. Pares, D. Reboulleau, P.-J. Royer, D. Fabre, E. Fadel, O. Mercier, S. Mussot, F. Stephan, P. Viard, J. Cerrina, P. Dorfmuller, S.M. Ghigna, Ph. Hervén, F. Le Roy Ladurie, J. Le Pavec, V. Thomas de Montpreville, L. Lamrani, Y. Castier, P. Cerceau, P. Augustin, S. Jean-Baptiste, S. Boudinet, P. Montravers, O. Brugière, G. Dauriat, G. Jébrak, H. Mal, A. Marceau, A.-C. Métivier, G. Thabut, E. Lhuillier, C. Dupin, V. Bunel, P. Falcoz, G. Massard, N. Santelmo, G. Ajob, O. Collange, O. Helms, J. Hentz, A. Roche, B. Bakouboula, T. Degot, A. Dory, S. Hirschi, S. Ohlmann-Caillard, L. Kessler, R. Kessler, A. Schuller, K. Bennedif, S. Vargas, J. Stauder, S. Ali-Azouaou, P. Bonnette, A. Chapelier, P. Puyo, E. Sage, J. Bresson, V. Caille, C. Cerf, J. Devaquet, V. Dumans-Nizard, M.-L. Felten, M. Fischler, A.-G. Si Larbi, M. Leguen, L. Ley, N. Liu, G. Trebbia, S. De Miranda, B. Douvry, F. Gonin, D. Grenet, A.M. Hamid, H. Neveu, F. Parquin, C. Picard, A. Roux, M. Stern, F. Bouillioud, P. Cahen, M. Colombat, C. Dautricourt, M. Delahousse, B. D'Urso, J. Gravisse, A. Guth, S. Hillaire, P. Honderlick, M. Lequintrec, E. Longchampt, F. Mellot, A. Scherrer, L. Temagoult, L. Tricot, M. Vasse, C. Veyrie, L. Zemoura, J. Berjaud, L. Brouchet, M. Dahan, F.O. Mathe, H. Benahoua, M. DaCosta, I. Serres, V. Merlet-Dupuy, M. Grigoli, A. Didier, M. Murris, L. Crognier, O. Fourcade, T. Krueger, H.B. Ris, M. Gonzalez, Ph. Jolliet, C. Marcucci, M. Chollet, F. Gronchi, C. Courbon, C. Berutto, O. Manuel, A. Koutsokera, J.-D. Aubert, L.P. Nicod, S. Mouraux, E. Bernasconi, C. Pattaroni, B.J. Marsland, P.M. Soccal, T. Rochat, L.M. Lücker, S. Hillinger, I. Inci, W. Weder, R. Schuepbach, M. Zalunardo, C. Benden, M.M. Schuurmans, A. Gaspert, D. Holzmann, N. Müller, C. Schmid, B. Vrugt, A. Fritz, D. Maier, K. Deplanche, D. Koubi, F. Ernst, T. Paprotka, M. Schmitt, B. Wahl, J.-P. Boissel, G. Olivera-Botello, C. Trocmé, B. Toussaint, S. Bourgoin-Voillard, M. Sève, M. Benmerad, V. Siroux, R. Slama, C. Auffray, D. Charron, D. Lefaudeux, J. Pellet
Přispěvatelé: Division of Pulmonary Medicine, Medical University Vienna, Clinique Universitaire de Pneumologie, Pôle Thorax et Vaisseaux, Centre Hospitalier Universitaire de Grenoble, Université Grenoble Alpes - UFR Médecine (UGA UFRM), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), unité de recherche de l'institut du thorax UMR1087 UMR6291 (ITX), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de Pneumologie, Nouvel Hôpital Civil Strasbourg, Division Pulmonary Medicine, University hospital of Zurich [Zurich], Geneva University Hospital (HUG), Rochat, Thierry, Licker, Marc
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
THP-DM
THP1-derived macrophages
Microbiota/immunology
Anabolism
Fibroblasts/immunology/pathology
[SDV]Life Sciences [q-bio]
Bacteria/classification/immunology
Matrix metalloproteinase
SPP1
Secreted phosphoprotein 1
Immunology and Allergy
Lung
Lung/immunology/microbiology/pathology
ddc:616
medicine.diagnostic_test
Microbiota
Airway remodeling
respiratory system
Middle Aged
3. Good health
Extracellular Matrix
macrophages
rRNA
Ribosomal RNA
Extracellular Matrix/immunology/pathology
Female
PDGFD
Platelet-derived growth factor D
THBS1
Thrombospondin 1
Lung Transplantation
Signal Transduction
Airway Remodeling/immunology
CFU
Colony-forming unit
Adult
BAL
Bronchoalveolar lavage
Immunology
COPD
Chronic obstructive pulmonary disease
MMP
Matrix metallopeptidase
Biology
Macrophages/immunology/pathology
KEGG
Kyoto Encyclopedia of Genes and Genomes
CHI3L1
Article
Proinflammatory cytokine
03 medical and health sciences
CLAD
Chronic lung allograft dysfunction
fibroblasts
Thrombospondin 1
GO
Gene Ontology
medicine
microbiota
Humans
IGF
Insulin-like growth factor
Thrombospondin
Signal Transduction/immunology
Matrix
Bacteria
Catabolism
Macrophages
IQR
Interquartile range
Fibroblasts
matrix
respiratory tract diseases
030104 developmental biology
Bronchoalveolar lavage
CHI3L1
Chitinase 3-like 1
SysCLAD
System prediction of Chronic Lung Allograft Dysfunction
KO
KEGG ortholog
Zdroj: Journal of Allergy and Clinical Immunology
Journal of Allergy and Clinical Immunology, Elsevier, 2018, Equipe III, 141 (2), pp.718--729.e7. ⟨10.1016/j.jaci.2017.06.022⟩
The Journal of Allergy and Clinical Immunology
Journal of Allergy and Clinical Immunology, Vol. 141, No 2 (2018) pp. 718-729.e7
ISSN: 0091-6749
Popis: Background Homeostatic turnover of the extracellular matrix conditions the structure and function of the healthy lung. In lung transplantation, long-term management remains limited by chronic lung allograft dysfunction, an umbrella term used for a heterogeneous entity ultimately associated with pathological airway and/or parenchyma remodeling. Objective This study assessed whether the local cross-talk between the pulmonary microbiota and host cells is a key determinant in the control of lower airway remodeling posttransplantation. Methods Microbiota DNA and host total RNA were isolated from 189 bronchoalveolar lavages obtained from 116 patients post lung transplantation. Expression of a set of 11 genes encoding either matrix components or factors involved in matrix synthesis or degradation (anabolic and catabolic remodeling, respectively) was quantified by real-time quantitative PCR. Microbiota composition was characterized using 16S ribosomal RNA gene sequencing and culture. Results We identified 4 host gene expression profiles, among which catabolic remodeling, associated with high expression of metallopeptidase-7, -9, and -12, diverged from anabolic remodeling linked to maximal thrombospondin and platelet-derived growth factor D expression. While catabolic remodeling aligned with a microbiota dominated by proinflammatory bacteria (eg, Staphylococcus, Pseudomonas, and Corynebacterium), anabolic remodeling was linked to typical members of the healthy steady state (eg, Prevotella, Streptococcus, and Veillonella). Mechanistic assays provided direct evidence that these bacteria can impact host macrophage-fibroblast activation and matrix deposition. Conclusions Host-microbes interplay potentially determines remodeling activities in the transplanted lung, highlighting new therapeutic opportunities to ultimately improve long-term lung transplant outcome.
Graphical abstract
Databáze: OpenAIRE
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