Inhibition of epidermal growth factor receptor stimulates prolactin expression in primary culture of the mouse pituitary gland
| Autor: | Hiroyuki Omori, Toshio Harigaya, Haruo Nogami, Yosuke Takei, Ryusuke Koshida, Masahiro Shibata |
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| Rok vydání: | 2019 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Pituitary gland endocrine system diseases Endocrinology Diabetes and Metabolism Primary Cell Culture 030209 endocrinology & metabolism Biology Mice 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Endocrinology Pregnancy Epidermal growth factor Internal medicine Gene expression medicine Animals Epidermal growth factor receptor Receptor Cells Cultured Mice Inbred ICR Epidermal Growth Factor Endocrine and Autonomic Systems Embryo Mammalian Somatotrophs Prolactin ErbB Receptors medicine.anatomical_structure Gene Expression Regulation Cell culture Pituitary Gland biology.protein Female Erlotinib hormones hormone substitutes and hormone antagonists 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Journal of Neuroendocrinology. 31 |
| ISSN: | 1365-2826 0953-8194 |
| DOI: | 10.1111/jne.12764 |
| Popis: | The roles of epidermal growth factor (EGF) in the regulation of prolactin (PRL) gene expression in the normal pituitary gland remain poorly understood. In the present study, the effects of EGF and an inhibitor of the EGF receptor, erlotinib, on PRL gene expression were examined both in the pituitary tumour cell line GH3 and in a primary culture of the mouse pituitary gland under similar experimental conditions. The results showed that EGF stimulated PRL expression in GH3 cells, but not in normal cells. Erlotinib was found to counteract EGF in GH3 cells inhibiting the PRL expression enhanced by EGF. By contrast, erlotinib induced an elevation in the PRL mRNA levels in the primary culture of the adult pituitary gland and the initiation of PRL production in the culture of the foetal pituitary gland in which PRL production had not yet occurred. Western blot analyses showed that EGF induced and erlotinib inhibited the activation of extracellular regulated protein kinase equally in GH3 and normal cells. These results suggest that the consequences of EGF receptor activation in normal PRL cells contradict those in adenomatous PRL cells. |
| Databáze: | OpenAIRE |
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