Changes in gene expression during male meiosis in Petunia hybrida
| Autor: | Hans de Jong, Marc Zabeau, Koen Weterings, Tom Gerats, Daphne Y. Rainey, Filip Cnudde, Veena Hedatale, Elisabeth S. Pierson, Janny L. Peters |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
genome-wide expression
DNA Complementary DNA Plant chromosome synapsis Molecular Plant Physiology arabidopsis-thaliana Biology Plant Genetics Laboratorium voor Erfelijkheidsleer Homology (biology) Transcriptome Meiosis Microspore Gene Expression Regulation Plant Gene expression Genetics Gene meiotic prophase Messenger RNA spermatogenic cells EPS-4 homolog fission yeast Reverse genetics Petunia spindle checkpoint Laboratory of Genetics protein Nucleic Acid Amplification Techniques Genome Plant transcriptional program |
| Zdroj: | Chromosome Research, 14(8), 919-932 Chromosome Research, 14, 8, pp. 919-932 Chromosome Research, 14, 919-932 Chromosome Research 14 (2006) 8 |
| ISSN: | 0967-3849 |
| DOI: | 10.1007/s10577-006-1099-5 |
| Popis: | We analyzed changes in gene expression during male meiosis in Petunia by combining the meiotic staging of pollen mother cells from a single anther with cDNA-AFLP transcript profiling of mRNA from the synchronously developing sister anthers. The transcript profiling experiments focused on the identification of genes with a modulated expression profile during meiosis, while premeiotic archesporial cells and postmeiotic microspores served as a reference. About 8000 transcript tags, estimated at 30% of the total transcriptome, were generated, of which around 6% exhibited a modulated gene expression pattern at meiosis. Cluster analysis revealed a transcriptional cascade that coincides with the initiation and progression through all stages of the two meiotic divisions. Fragments that exhibited high expression specifically during meiosis I were characterized further by sequencing; 90 out of the 293 sequenced fragments showed homology with known genes, belonging to a wide range of gene classes, including previously characterized meiotic genes. In-situ hybridization experiments were performed to determine the spatial expression pattern for five selected transcript tags. Its concurrence with cDNA-AFLP transcript profiles indicates that this is an excellent approach to study genes involved in specialized processes such as meiosis. Our data set provides the potential to unravel unique meiotic genes that are as yet elusive to reverse genetics approaches. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink