The Nuclear Uptake of Androgen by Human Benign Prostate in Vitro: Action of Antiandrogens
| Autor: | E.J.G. Milroy, W. I. P. Mainwaring, Elizabeth K. Symes |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Male
medicine.medical_specialty medicine.drug_class Antiandrogens Urology medicine.medical_treatment Diethylstilbestrol In Vitro Techniques Pharmacology Steroid Flutamide chemistry.chemical_compound Sex hormone-binding globulin Prostate Internal medicine medicine Humans Cell Nucleus biology business.industry Cyproterone acetate Androgen Antagonists Androgen Endocrinology medicine.anatomical_structure chemistry Androgens biology.protein business medicine.drug |
| Zdroj: | Journal of Urology. 120:180-183 |
| ISSN: | 1527-3792 0022-5347 |
| Popis: | An in vitro test system suitable to assess the potency of putative antiandrogens has been developed, using the human benign prostatic tissue obtained at operation. The system circumvents some problems associated with using human tissue, such as the presence of endogenous steroid and contamination with plasma proteins (particularly sex hormone binding globulin). Slices of tissue were incubated in the presence of 3H-testosterone and the uptake into nuclei was determined. The nature of the nuclear radioactivity and the steroid specificity indicates a mechanism similar to that established in the rat ventral prostate. The action of antiandrogens (cyproterone acetate, diethylstilbestrol, flutamide, hydroxylated flutamide and gestonorone capronate) has been studied at various concentrations. |
| Databáze: | OpenAIRE |
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