CircRNA circBACH1 (hsa_circ_0061395) serves as a miR-656–3p sponge to facilitate hepatocellular carcinoma progression through increasing SERBP1 expression
| Autor: | Peng Du, Jianpeng He, Yong Li, Guoyong Li |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Carcinoma Hepatocellular Biophysics Vimentin In Vitro Techniques Biochemistry Flow cytometry Mice 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Line Tumor microRNA medicine Animals Humans Gene silencing Molecular Biology Cell Proliferation Mice Inbred BALB C Gene knockdown biology medicine.diagnostic_test Chemistry Liver Neoplasms RNA-Binding Proteins RNA Circular Cell Biology medicine.disease Xenograft Model Antitumor Assays digestive system diseases Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Apoptosis 030220 oncology & carcinogenesis Hepatocellular carcinoma Disease Progression Cancer research biology.protein Female |
| Zdroj: | Biochemical and Biophysical Research Communications. 556:1-8 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2021.03.136 |
| Popis: | Hsa_circ_0061395(circBACH1) and SERBP1(SERPINE1 mRNA binding protein 1) have been reported to play a carcinogenic role in HCC.In this study, circBACH1, microRNA(miR)-656–3p, and SERBP1 expression levels with quantitative real-time polymerase chain reaction (qRT-PCR) in HCC tissue specimens and cells.The protein levels of SERBP1, E-Cadherin, vimentin, and N-Cadherin were detected with western blotting.Cell proliferation, migration, invasion, and apoptosis were determined with CCK-8, colony formation, transwell, and flow cytometry assays.The targeting relatio-nship between circBACH1 or SERBP1 and miR-656–3p was verified by dual-lucifer- ase reporter assay.The role of circBACH1 was validated by xenograft assay.CircBAC- H1 and SERBP1 were upregulated in HCC tissues and cells.Both circBACH1 and SERBP1 knockdown constrained proliferation, migration, invasion, and EMT(epithel-ial-mesenchymal transition), and facilitated apoptosis of HCC cells in vitro.Knockdo-wn of circBACH1 reduced HCC growth in vivo. SERBP1 overexpression partially neutralized the repressive effect of circBACH1 silencing on malignant behaviors of HCC cells.CircBACH1 sponged miR-656–3p to elevate SERBP1 expression, thereby accelerating the progression of HCC.The research provided a new evidence to support the role of circBACH1 in HCC. |
| Databáze: | OpenAIRE |
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