Effect of urine storage on urinary uric acid concentrations
| Autor: | Tetsuya Yamamoto, Jun-ichi Yamakita, Zenta Tsutsumi, Toshikazu Hada, Yuji Moriwaki, Sumio Takahashi |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
Chromatography Gout Chemistry Urinary system Clinical Biochemistry nutritional and metabolic diseases General Medicine Metabolism Urine urologic and male genital diseases medicine.disease Specimen Handling Uric Acid Dilution Uric acid crystals chemistry.chemical_compound Biochemistry Reference Values medicine Humans Uric acid Hyperuricemia |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 37:355-359 |
| ISSN: | 1758-1001 0004-5632 |
| DOI: | 10.1258/0004563001899276 |
| Popis: | Accurate determination of serum and urinary uric acid concentrations is essential for the diagnosis and classification of gout according to uric acid metabolism derangement. Urine and/or serum samples are often kept at either 4°C or 20°C until assayed, when a large number of samples are handled simultaneously. Our preliminary study indicated a significant decrease in urinary uric acid concentration after preservation, regardless of the storage temperature. Uric acid crystals were often observed in these cases which showed a marked decrease in urinary uric acid concentration after storage. In the present study, we sought the factor(s) that might cause this decrease in urinary uric acid concentration, as well as measures to overcome the problem. High urinary uric acid concentration and low pH proved to play major roles in the decrease in urinary uric acid concentration after storage. In contrast, dilution of the urine samples before storage resulted in no significant change in urinary uric acid concentration. Based on these results, we recommend diluting urine before storage for determination of uric acid concentration and avoiding underestimation. |
| Databáze: | OpenAIRE |
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