Analysis of the secondary structure that negatively regulates inducible cat translation by use of chemical probing and mutagenesis
| Autor: | Zhiping Gu, Robert Harrod, Paul S. Lovett |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Terminator Regions Genetic Messenger RNA Base Sequence Molecular Sequence Data Mutagenesis RNA Translation (biology) General Medicine Biology Molecular biology Ribosome Chloramphenicol Gene Expression Regulation Protein Biosynthesis Gene expression Genetics Nucleic Acid Conformation Coding region RNA Messenger Gene |
| Zdroj: | Gene. 140:79-83 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(94)90734-x |
| Popis: | Induction of chloramphenicol (Cm) acetyltransferase-encoding genes (cat) by Cm has been proposed to result from the destabilization of a stem-loop that sequesters the ribosome-binding site for the cat coding sequence. Destabilization is caused by the stalling of a ribosome at a specific site in the leader of cat transcripts that immediately precedes the stem-loop. By use of in vivo dimethylsulfate probing of cat-86 leader mRNA, we demonstrate the existence of the stemloop structure in cat transcripts isolated from uninduced cells and its release during induction. Leader mutations chosen to provide the mRNA with an alternative folding pattern that destabilizes the stem-loop cause constitutive cat expression. Our results establish the occurrence in vivo of the stem-loop in cat-86 transcripts and its role as a negative regulator of cat expression. |
| Databáze: | OpenAIRE |
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