Protective Effects of Hyperoxygenated Solution Preconditioning on Intestinal Ischemia–Reperfusion Injury in Rabbits
| Autor: | Guihe Zhang, Changjun Gao, Xude Sun, Lichun Han, Lixian Xu, Hui Zhang, Wei Chai |
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| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Superoxide dismutase Lesion chemistry.chemical_compound medicine.artery Internal medicine medicine Animals Superior mesenteric artery Intestinal Mucosa Ischemic Preconditioning Saline chemistry.chemical_classification Lagomorpha biology Histocytochemistry Superoxide Dismutase business.industry Glutathione peroxidase Catalase biology.organism_classification medicine.disease Malondialdehyde Intestines Oxygen Solutions Endocrinology chemistry Reperfusion Injury Anesthesia biology.protein Surgery Rabbits medicine.symptom business Reperfusion injury |
| Zdroj: | Journal of Surgical Research. 135:268-274 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2006.03.019 |
| Popis: | Background The aim of this study was to investigate the protective effects of hyperoxygenated solution (HOS) preconditioning on intestinal ischemia–reperfusion (IR) injury in rabbits. Materials and methods Thirty-two rabbits were randomly divided into four groups as follows: (1) control group in which sham operation was performed (Sham group); (2) sham operation and HOS treatment group (sham+H group); (3) ischemia–reperfusion group (IR group); (4) ischemia–reperfusion and HOS treatment group (H group). Intestinal IR model was produced by clamping superior mesenteric artery with an atraumatic vascular clamp for 1 h, followed by reperfusion for 2 h. Animals in H group received intravenous HOS infusion (20 mL/kg) every day for 5 days before ischemia–reperfusion; animals in the sham+H group received the same amount of HOS before sham operation, and animals in IR group received the same amount of normal saline in the same way. At the end of reperfusion, histopathological changes of intestine were observed, and malondialdehyde (MDA) levels, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities in intestinal tissues were also detected. Intestinal barrier function was assessed by blood d -lactate levels and bacterial translocation (BT). Results The H group showed significantly lower MDA levels and higher activities of SOD, CAT, and GSH-Px in the intestinal tissue compared with the IR group. Furthermore, the mean d -lactate levels and incidence of BT in the H group were significantly lower than those in the IR group. Histopathological analysis also indicated that there were significant histological improvements in the H group compared with the IR group. Conclusions HOS preconditioning at an appropriate dose ameliorates the deleterious changes in intestinal mucosal injury and barrier function associated with IR by effectively preventing a decrease in the intestinal antioxidant defense system, which is another simple and effective measure to protect intestine from IR injury. |
| Databáze: | OpenAIRE |
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