The degradation of chlorophyll pigments in dairy silage: the timeline of anaerobic fermentation
| Autor: | Mark A. Rasmussen, Jacob W. Petrich, Anthony Song, Kalyan Santra |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Chlorophyll
Pheophytin Rumen 030309 nutrition & dietetics Silage Zea mays 03 medical and health sciences chemistry.chemical_compound Phytol Pigment 0404 agricultural biotechnology Animals Anaerobiosis Food science Magnesium ion 0303 health sciences Nutrition and Dietetics Spectrophotometry Atomic food and beverages 04 agricultural and veterinary sciences Animal Feed 040401 food science Lactic acid Milk chemistry visual_art Fermentation visual_art.visual_art_medium Cattle Digestion Female Agronomy and Crop Science Medicago sativa Food Science Biotechnology |
| Zdroj: | Journal of the Science of Food and Agriculture. 101:2863-2868 |
| ISSN: | 1097-0010 0022-5142 |
| DOI: | 10.1002/jsfa.10917 |
| Popis: | Background Detection of chlorophyll metabolites in milk has recently been suggested to be an indicator of a grass-fed diet fed for cattle. Such a means of detection, however, is complicated when the grazing season is over because cattle can be fed fermented silage ingredients, such as alfalfa and corn silage. During fermentation, chlorophyll compounds and other pigments undergo degradation due to the accumulation of lactic acid and the resultant decline in pH. Results We monitored degradation of chlorophyll compounds by measuring the fluorescence and absorption spectra of silage extracts. The spectroscopic evidence supports the hypothesis that chlorophylls are converted into fluorescent products, such as pheophytin, and further cleaved into pheophorbide. The degradation starts with dechelation and removal of the magnesium ion to produce pheophytin. Further removal of the phytol chain from pheophytin results in the production of pheophorbide. Conclusions The fluorescence intensity of these degradation products is reduced compared to that of the parent molecule. These findings are important in understanding the fluorescent signal in milk when cows consume silage rather than fresh pasture grass. © 2020 Society of Chemical Industry. |
| Databáze: | OpenAIRE |
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