Strain-level bacterial identification by CeO2-catalyzed MALDI-TOF MS fatty acid analysis and comparison to commercial protein-based methods
| Autor: | Nicholas R. Saichek, Kent J. Voorhees, Christopher Cox, Kirk R. Jensen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Proteomics
Mass spectrometry Catalysis Article 03 medical and health sciences Metabolomics Bacterial Proteins 030304 developmental biology chemistry.chemical_classification 0303 health sciences Multidisciplinary biology Bacteria 030306 microbiology Fatty Acids Bacterial taxonomy Fatty acid Reproducibility of Results Cerium biology.organism_classification Enterobacteriaceae Matrix-assisted laser desorption/ionization chemistry Biochemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep10470 |
| Popis: | Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as a rapid approach for clinical bacterial identification. However, current protein-based commercial bacterial ID methods fall short when differentiating closely related species/strains. To address this shortcoming, we employed CeO2-catalyzed fragmentation of lipids to produce fatty acids using the energy inherent to the MALDI laser as a novel alternative to protein profiling. Fatty acid profiles collected from Enterobacteriaceae, Acinetobacter and Listeria using CeO2-catalyzed metal oxide laser ionization (MOLI MS), processed by principal component analysis and validated by leave–one-out cross-validation (CV), showed 100% correct classification at the species level and 98% at the strain level. In comparison, protein profile data from the same bacteria yielded 32%, 54% and 67% mean species-level accuracy using two MALDI-TOF MS platforms, respectively. In addition, several pathogens were misidentified by protein profiling as non-pathogens and vice versa. These results suggest novel CeO2-catalyzed lipid fragmentation readily produced (i) taxonomically tractable fatty acid profiles by MOLI MS, (ii) highly accurate bacterial classification and (iii) consistent strain-level ID for bacteria that were routinely misidentified by protein-based methods. |
| Databáze: | OpenAIRE |
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