Redistribution of a murine humoral immune response following removal of an immunodominant B cell epitope from a recombinant fusion protein
| Autor: | Lea Brys, Jean-Pierre Y. Scheerlinck, Robert Deleys, Anja B. Geldhof, Eric Saman, Patrick De Baetselier |
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| Rok vydání: | 1993 |
| Předmět: |
medicine.drug_class
Recombinant Fusion Proteins Immunology Molecular Sequence Data chemical and pharmacologic phenomena Immunodominance Biology HIV Antibodies Monoclonal antibody Epitope Epitopes Mice Structure-Activity Relationship Viral Proteins Antigen Antibody Specificity medicine Animals Viral Regulatory and Accessory Proteins Amino Acid Sequence Molecular Biology B-Lymphocytes Mice Inbred BALB C Linear epitope Base Sequence Immunogenicity Antibodies Monoclonal beta-Galactosidase Molecular biology Fusion protein HIV Envelope Protein gp41 DNA-Binding Proteins Repressor Proteins Antibody Formation biology.protein bacteria Female Antibody |
| Zdroj: | Molecular immunology. 30(8) |
| ISSN: | 0161-5890 |
| Popis: | Immunization of different mice strains with a recombinant fusion protein composed of the vector-encoded N-terminal leader peptide CroLac (containing lambda Cro and LacI fragments) and a part of the transmembrane protein of HIV-1 (gp41) led to a high anti-CroLac humoral immune response. A detailed analysis of this response revealed the presence of an immunodominant, linear B cell epitope localized near the C-terminus of the CroLac fragment. The immune response seemed to be biased towards this epitope since few or no monoclonal antibodies (mAb) could be generated against the remaining part of CroLac and the gp41 fragment. Upon removal of the immunodominant region from the fusion protein the immune response was redirected and spread over the previously non-immunogenic regions. Consequently, we report a model system in which an immunodominant B cell epitope biases the immune response away from less immunogenic epitopes on the same molecule. |
| Databáze: | OpenAIRE |
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