Sensitive identification of neoantigens and cognate TCRs in human solid tumors

Autor:	Brian Stevenson, Marion Arnaud, Michal Bassani-Sternberg, Vincent Zoete, Florian Huber, Blanca Navarro Rodrigo, Marta A S Perez, Raphael Genolet, Petra Baumgaertner, Chloe Chong, Julien Schmidt, Lana E. Kandalaft, Philippe Guillaume, Sara Bobisse, Alexandre Harari, Johanna Chiffelle, Morgane Magnin, Melita Irving, Daniel E. Speiser, George Coukos, David Gfeller, Tu Nguyen-Ngoc
Rok vydání:	2021
Předmět:	Animals Antigens Neoplasm/genetics CD8-Positive T-Lymphocytes Humans Lymphocytes Tumor-Infiltrating Mice Neoplasms/genetics Neoplasms/therapy Receptors Antigen T-Cell/genetics T-Lymphocytes Receptors Antigen T-Cell Biomedical Engineering Bioengineering Biology Applied Microbiology and Biotechnology 03 medical and health sciences 0302 clinical medicine Antigen Antigens Neoplasm Neoplasms 030304 developmental biology 0303 health sciences T-cell receptor Tumor antigen 3. Good health Rare tumor 030220 oncology & carcinogenesis Cancer research Molecular Medicine Identification (biology) Biotechnology
Zdroj:	Nature biotechnology, vol. 40, no. 5, pp. 656-660
ISSN:	1546-1696 1087-0156
DOI:	10.1038/s41587-021-01072-6
Popis:	The identification of patient-specific tumor antigens is complicated by the low frequency of T cells specific for each tumor antigen. Here we describe NeoScreen, a method that enables the sensitive identification of rare tumor (neo)antigens and of cognate T cell receptors (TCRs) expressed by tumor-infiltrating lymphocytes. T cells transduced with tumor antigen-specific TCRs identified by NeoScreen mediate regression of established tumors in patient-derived xenograft mice.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83e3c6bf7513b7d8f19e6fefcf9aa0e4 https://doi.org/10.1038/s41587-021-01072-6 Zobrazit plný text záznamu Full text from SpringerLink