The influence of inulin on the absorption of nitrogen and the production of metabolites of protein fermentation in the colon
| Autor: | Pieter Evenepoel, Yvo Ghoos, Bert Bammens, Vicky De Preter, Anja Luypaerts, Karel Geboes, Paul Rutgeerts, Karen Geboes, Gert De Hertogh, Kristin Verbeke |
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| Rok vydání: | 2006 |
| Předmět: |
Male
LACTULOSE DIETARY FIBER Metabolite Egg protein Medicine (miscellaneous) Lactose Pilot Projects BREATH TEST TRANSIT-TIME Cresols Feces chemistry.chemical_compound Nutrition and Dietetics INTESTINAL BACTERIA Inulin HUMANS OLIGOSACCHARIDES Biochemistry Isotope Labeling Female Dietary Proteins colonic nitrogen Life Sciences & Biomedicine Adult Colon Nitrogen EGG PROTEIN stable isotopes Statistics Nonparametric Excretion Ammonia Dietary Carbohydrates Carbohydrate fermentation Humans OLIGOFRUCTOSE AMMONIA Analysis of Variance Science & Technology Nutrition & Dietetics Nitrogen Isotopes Carbohydrate carbohydrate fermentation Intestinal Absorption chemistry Fermentation Tyrosine |
| Zdroj: | British Journal of Nutrition. 96:1078-1086 |
| ISSN: | 1475-2662 0007-1145 |
| DOI: | 10.1017/bjn20061936 |
| Popis: | In the present study, the production and fate of bacterial metabolites in the colon were investigated in a direct way using two substrates labelled with stable isotopes: lactose [15N,15N]ureide as a source of labelled ammonia and egg proteins intrinsically labelled with [2H4]tyrosine as a precursor of [2H4]p-cresol. Both ammonia and phenolic compounds are believed to be carcinogenic. Stimulation of carbohydrate fermentation in order to prevent accumulation of these toxic metabolites was induced by inclusion of inulin in a test meal or by addition of inulin to the daily diet, allowing us to distinguish between changes induced by the actual presence of a fermentable carbohydrate and effects caused by a long-term dietary intervention. When a single dose of inulin was administered together with the labelled substrates, a significant increase in faecal15N excretion, accompanied by a proportional decrease in urinary15N excretion was observed, probably reflecting an enhanced uptake of ammonia for bacterial biosynthesis, since an increased concentration of labelled N in bacterial pellets was found. A statistically significant reduction of urinary [2H4]p-cresol excretion was also noted. Upon supplementation of inulin to the daily diet during 4 weeks, however, only a tendency towards decreased urinary excretion of both labelled and unlabelledp-cresol was noted. Further studies are warranted to confirm these results in a larger cohort. |
| Databáze: | OpenAIRE |
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