Increased 12-HETE production in bovine lymphocytes during selenium deficiency
| Autor: | George Hildenbrandt, Yu Zhang Cao, C. Channa Reddy, Richard W. Scholz, Andrea M. Mastro, Jane F. Maddox |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Lymphocyte Lipoxygenase Biophysics Arachidonic Acids Lymphocyte proliferation Lymphocyte Activation Biochemistry Mass Spectrometry Selenium chemistry.chemical_compound Selenium deficiency Internal medicine Hydroxyeicosatetraenoic Acids medicine Animals 12-Hydroxy-5 8 10 14-eicosatetraenoic Acid Lymphocytes Molecular Biology Calcimycin biology Cell growth Cell Biology Metabolism medicine.disease Endocrinology medicine.anatomical_structure chemistry Concanavalin A Cell culture cardiovascular system biology.protein Cattle Female lipids (amino acids peptides and proteins) Arachidonic acid |
| Zdroj: | Biochemical and Biophysical Research Communications. 181:389-395 |
| ISSN: | 0006-291X |
| Popis: | When peripheral blood lymphocytes were incubated with arachidonic acid in the presence of Ca++ ionophore (A23187), the cells from the selenium-deficient dairy cows produced significantly greater quantities of 12-hydroxyeicosatetraenoic acid (12-HETE) than the cells from the selenium-supplemented animals. The major product of reaction was verified as 12-HETE by cochromatography with a 12-HETE standard on an HPLC and structural analysis by GC-MS. Additionally, concanavalin A-stimulated lymphocyte proliferation was significantly decreased in cells from the Se-deficient cows. Furthermore, 12-HETE generated by the A23187-stimulated lymphocytes inhibited lymphocyte proliferation when added to Se-supplemented cell cultures. These observations suggest that self-regulation of lymphocyte proliferation might be mediated by 12-HETE production, especially during an altered nutritional state such as Se deficiency. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|