An estimate of the number of hepatocyte donors required to provide reasonable estimates of human hepatic clearance from in vitro experiments
| Autor: | Heather Eng, Ritwik Ghosh, Paul M. Silber, James P. Byers, Genfu Chen, Donald B. White, Anand Katta, Nicola J. Hewitt, Kenneth Bachmann |
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| Rok vydání: | 2009 |
| Předmět: |
Cryopreservation
Biochemistry (medical) Clinical Biochemistry Pharmaceutical Science Hepatic clearance Biology Models Biological In vitro Confidence interval Tissue Donors Andrology medicine.anatomical_structure Pharmaceutical Preparations In vivo Hepatocyte Immunology medicine Hepatocytes Humans Pharmacology (medical) |
| Zdroj: | Drug metabolism letters. 1(2) |
| ISSN: | 1874-0758 |
| Popis: | Cryopreserved human hepatocytes in suspension were used to estimate in vivo hepatic clearances for six different drugs. In vitro intrinsic clearances were measured on the basis of substrate depletion. The number of different hepatocyte donors required for a reasonable estimate of in vivo hepatic clearance-within twice or 1 / 2 of the actual value — was determined. Depending upon the desired level of confidence, anywhere from 9-20 donors are required by this method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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