Scanning Electron Microscopy Of Uncoated Human Metaphase Chromosomes
| Autor: | L. F.-H. Lam, L. R. Sweney, Burton L. Shapiro |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Histology
Materials science Morphology (linguistics) Scanning electron microscope Gold coating Substrate (electronics) Chromatids Pathology and Forensic Medicine Hydroxides Chromosomes Human Humans Composite material Metaphase Mitosis FOIL method Hydrolyzable Tannins Chromosome Banding Silver mirror Quaternary Ammonium Compounds Crystallography Glutaral Microscopy Electron Scanning Silver Nitrate Gold Aluminum |
| Zdroj: | Journal of Microscopy. 115:151-160 |
| ISSN: | 0022-2720 |
| DOI: | 10.1111/j.1365-2818.1979.tb00164.x |
| Popis: | SUMMARY Human metaphase chromosomes were processed with a 3% glutaraldehyde-tannic acid technique and examined in a scanning electron microscope at 20 kV either without added metal coating or with 2 nm of sputtered gold coating. Several substrates—aluminium foil, silver mirror deposit and sputtered gold—provided good conductive backgrounds for chromosomal spreads. Silver mirror deposit was the best conductive substrate tested. This method should prove to be a useful tool for monitoring the three-dimensional morphology of mitotic chromosomes with the possibility of studying various banding techniques, chromosomal uncoiling and secondary constrictions currently being examined in chromosomal studies. |
| Databáze: | OpenAIRE |
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