Induction of the Cpx Envelope Stress Pathway Contributes to Escherichia coli Tolerance to Antimicrobial Peptides
| Autor: | Bianca Audrain, Christophe Beloin, Jean-Yves Coppée, Amira Zairi, Jean-Marc Ghigo, Lionel Ferrières, Curtis B. Dobson, Guillaume Soubigou |
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| Přispěvatelé: | Cellule Pasteur, Université Paris Diderot - Paris 7 (UPD7)-PRES Sorbonne Paris Cité, Génétique des Biofilms, Institut Pasteur [Paris] (IP), Faculté de médecine de Sousse [Ibn EL Jazzar], Transcriptome et Epigénome (PF2), Faculty of Life Sciences [Manchester], University of Manchester [Manchester], This work was supported by a grant from the European Commission (grant LSHE-CT-2006-037692) and the French Government's Investissement d'Avenir program Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant ANR-10-LABX-62-IBEID)., We thank Sylvie Létoffé for helpful discussions. We also thank Mike Birch, Ai2 Ltd., as well as members of the NPARI consortium for support during the course of this project., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 037692, European STREP 'NPARI', Institut Pasteur [Paris] |
| Rok vydání: | 2013 |
| Předmět: |
MESH: Apolipoproteins E/*metabolism
MESH: Signal Transduction Antimicrobial peptides Genetics and Molecular Biology Peptide Biology medicine.disease_cause Applied Microbiology and Biotechnology Melittin Microbiology MESH: Gene Expression Profiling 03 medical and health sciences chemistry.chemical_compound Apolipoproteins E Stress Physiological Escherichia coli medicine Humans MESH: Escherichia coli/*drug effects/*physiology MESH: *Stress 030304 developmental biology chemistry.chemical_classification 0303 health sciences MESH: Humans Dermaseptin MESH: Anti-Bacterial Agents/*metabolism Ecology 030306 microbiology Gene Expression Profiling Drug Tolerance Antimicrobial [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Peptide Fragments Anti-Bacterial Agents chemistry MESH: *Drug Tolerance Signal transduction Metabolic Networks and Pathways MESH: Metabolic Networks and Pathways/genetics Polymyxin B Signal Transduction MESH: Peptide Fragments/*metabolism Food Science Biotechnology medicine.drug |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, 2013, 79 (24), pp.7770-7779. ⟨10.1128/AEM.02593-13⟩ Applied and Environmental Microbiology, American Society for Microbiology, 2013, 79 (24), pp.7770-7779. ⟨10.1128/AEM.02593-13⟩ |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | Antimicrobial peptides produced by multicellular organisms as part of their innate system of defense against microorganisms are currently considered potential alternatives to conventional antibiotics in case of infection by multiresistant bacteria. However, while the mode of action of antimicrobial peptides is relatively well described, resistance mechanisms potentially induced or selected by these peptides are still poorly understood. In this work, we studied the mechanisms of action and resistance potentially induced by ApoEdpL-W, a new antimicrobial peptide derived from human apolipoprotein E. Investigation of the genetic response of Escherichia coli upon exposure to sublethal concentrations of ApoEdpL-W revealed that this antimicrobial peptide triggers activation of RcsCDB, CpxAR, and σ E envelope stress pathways. This genetic response is not restricted to ApoEdpL-W, since several other antimicrobial peptides, including polymyxin B, melittin, LL-37, and modified S 4 dermaseptin, also activate several E. coli envelope stress pathways. Finally, we demonstrate that induction of the CpxAR two-component system directly contributes to E. coli tolerance toward ApoEdpL-W, polymyxin B, and melittin. These results therefore show that E. coli senses and responds to different antimicrobial peptides by activation of the CpxAR pathway. While this study further extends the understanding of the array of peptide-induced stress signaling systems, it also provides insight into the contribution of Cpx envelope stress pathway to E. coli tolerance to antimicrobial peptides. |
| Databáze: | OpenAIRE |
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