Induction of hypoxia inducible factor 1 by oxygen glucose deprivation is attenuated by hypoxic preconditioning in rat cultured neurons
| Autor: | Ulrich Dirnagl, Linda Iurato, N. K. Isaev, Andreas Meisel, Markus Weih, Karsten Ruscher, George Trendelenburg |
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| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Hypoxia-Inducible Factor 1 Time Factors Ischemia Biology Internal medicine Conditioning Psychological medicine Animals Hypoxia Transcription factor Cells Cultured Cerebral Cortex Neurons General Neuroscience Nuclear Proteins DNA Hypoxia (medical) Hypoxia-Inducible Factor 1 alpha Subunit medicine.disease Privation Rats DNA-Binding Proteins Tolerance induction Glucose medicine.anatomical_structure Endocrinology nervous system Hypoxia-inducible factors Neuron medicine.symptom Transcription Factors |
| Zdroj: | Neuroscience Letters. 254:117-120 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/s0304-3940(98)00688-0 |
| Popis: | Hypoxia-inducible factor 1 (HIF-1) is a posttranscriptionally regulated transcription factor, controlling several hypoxia-inducible genes. Here we show a rapid and transient increase of HIF-1 DNA binding activity in a dose dependent manner in primary cortical neurons of rats exposed to oxygen glucose deprivation (OGD) for 30, 60, 90, or 120 min. A reduced induction of binding activity was observed when neurons were preconditioned by a non-lethal OGD interval (60 min) 48 h prior to the 90 min OGD. Thus, hypoxic preconditioning reduces activation of HIF-1 binding activity. The molecular mechanism of HIF-1 activation, its target genes, and its role in tolerance induction and/or ischemia induced damage remain to be elucidated. |
| Databáze: | OpenAIRE |
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