Cytotoxicity and hemolysis by an extract of the sponge Pachymatisma johnstonii
| Autor: | L. Guimbretière, P. Moré, G. Bourget, J. Le Boterff, Jean-François Verbist, M.T. Moré |
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| Rok vydání: | 1988 |
| Předmět: |
Cell Survival
In Vitro Techniques Biology Toxicology Hemolysis Dithiothreitol chemistry.chemical_compound medicine Animals Humans Cytotoxicity Hydrogen-Ion Concentration medicine.disease Trypsin biology.organism_classification In vitro Porifera Sponge Red blood cell Papain medicine.anatomical_structure chemistry Biochemistry Chromatography Gel Marine Toxins medicine.drug |
| Zdroj: | Toxicon. 26:324-327 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/0041-0101(88)90224-3 |
| Popis: | G. Bourget , M. T. More , P. More , L. Guimbretiere , J. Le Boterff and J. F. Verbist . Cytotoxicity and hemolysis by an extract of the sponge Pachymatisma johnstonii. Toxicon 26, 324 – 327, 1988 — In vitro cytotoxicity on human rhinopharynx KB cells, as well as hemolytic activity on human erythrocytes, was produced by an aqueous extract of the sponge Pachymatisma johnstonii. Optimum temperature and pH were the same for both activities (37°C, pH 5). Moreover, after partial purification, the compounds involved were found in the same chromatographic fraction. These compounds were sensitive to treatment with dithiothreitol, but not with papain or trypsin. Cytotoxicity was destroyed by heat, whereas hemolysis subsisted after the extract was heated to 100°C. |
| Databáze: | OpenAIRE |
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