DNA replication of wheat dwarf virus, a geminivirus, requires two cis-acting signals
| Autor: | Bruno Gronenborn, Matthias Kammann, Volker Matzeit, Sabine Schaefer, Hans-Joachim Schalk, Jeff Schell |
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| Rok vydání: | 1991 |
| Předmět: |
DNA Replication
Genetics DNA Mutational Analysis Restriction Mapping DNA replication Regulatory Sequences Nucleic Acid Biology Virus Replication Origin of replication biology.organism_classification Genome Virology Virus Plant Viruses Blotting Southern chemistry.chemical_compound Restriction map Viral replication chemistry DNA Viral Wheat dwarf virus Chromosome Deletion Triticum DNA |
| Zdroj: | Virology. 184:786-790 |
| ISSN: | 0042-6822 |
| Popis: | Replication of the single-stranded DNA genome of wheat dwarf virus (WDV) leads to the accumulation of covalently closed double-stranded DNA of genome length in infected cells. By studying the replication properties of a naturally occurring deletion mutant of WDV isolated from infected plants and of deletion mutants constructed in vitro, we have defined cis-acting regions required for viral DNA replication. The results show that two distinct regions are required in cis to yield the normal replicative forms of WDV-DNA. |
| Databáze: | OpenAIRE |
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